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General Information
Symbol
Dmel\Sec71DN.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0326753
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of a dominant negative form of Sec71 (carries the amino acid replacement E677K).

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The expression of Sec71DN.Scer\UAS under the combined control of Scer\GAL4GSG2295 and RU486 treatment from mid-third instar larval stage onwards leads to dorsal multidendritic ddaC neurons exhibiting a fully penetrant defect in dendrite pruning 16h-24h after puparium formation, as shown by the persistence of some dendritic branches, but exhibiting no obvious defects in dendritic arborization at the white pupa stage, and leads to no defects in the elimination of ddaF neurons by apoptosis at 16h after puparium formation, as compared to controls; at wandering third instar larval stage, this expression leads to dorsal multidendritic ddaC neurons exhibiting Golgi apparatus defects, as shown by the severe decrease in the number of GalT or GM130 punctate structures in the soma, and exhibiting decreased protein secretion, but exhibiting no obvious defects in ER exit sites, as shown by the apparently normal pattern of Sec31 punctate structures in the soma, as compared to controls.

Clonal expression under the control of Scer\GAL4109(2)80 leads to dorsal multidendritic ddaD/E neurons exhibiting defective pruning, as shown by the persistence of some dendrites at 19h after puparium formation, as compared to controls.

Expression under the control of Scer\GAL4ppk.PU leads a simplified dendritic tree in dorsal multidendritic ddaC neurons at the the white pupa stage, as compared to controls.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Sec71DN.Scer\UAS
Sec71DN.UAS
Name Synonyms
Secondary FlyBase IDs
    References (2)