FB2021_02, released April 13, 2021

Allele: Scer\GAL4DBD.R11F02

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General Information
Symbol
Scer\GAL4DBD.R11F02
Species
S. cerevisiae
Name
FlyBase ID
FBal0326997
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
Carried in Construct
P{R11F02-GAL4.DBD}
Also Known As
R11F02-DBD
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
in vitro construct - coding region fusion
(Heckscher et al., 2015)
in vitro construct - regulatory fusion
(Heckscher et al., 2015)
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
       
      Progenitor genotype
      Carried in construct
      P{R11F02-GAL4.DBD}
      Cytology
      Nature of the lesion
      Statement
      Reference

      The R11F02 enhancer fragment and Drosophila synthetic core promoter (DSCP) drive expression of the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain). A Hsp70 transcriptional terminator is also present.

      (Heckscher et al., 2015)
      Allele components
      Product class / Tool use(s)
      Encoded product / tool
      GAL4(DBD)::Zip-
      Associated Sequence Features
      GMR11F02
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      first instar larval stage
      larval A08c projection neuron

      Comment: when combined with P{eve-p65.EL.AD}

      (Heckscher et al., 2015)
      larval A08e1 local neuron

      Comment: when combined with P{eve-p65.EL.AD}

      (Heckscher et al., 2015)
      larval A08e2 local neuron

      Comment: when combined with P{eve-p65.EL.AD}

      (Heckscher et al., 2015)
      larval A08e3 local neuron

      Comment: when combined with P{eve-p65.EL.AD}

      (Heckscher et al., 2015)
      larval A08s projection neuron

      Comment: when combined with P{eve-p65.EL.AD}

      (Heckscher et al., 2015)
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Optogenetic activation (using Cnoc\ChR1Cs.IVS.20xUAS.Venus with red light) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R11F02 and Hsap\RELAAD.eve.EL in larvae causes bending of the body (C-bends) during crawling, with significantly greater head and tail angles than controls.

      (Heckscher et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Scer\GAL4DBD.R11F02.T:Zzzz\Zip-
      Scer\GAL4DBD.R11F02
      Name Synonyms
      Secondary FlyBase IDs
        References (4)