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General Information
Symbol
Hsap\MARK1UAS.Tag:MYC,Tag:HA
Species
H. sapiens
Name
FlyBase ID
FBal0327435
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of Hsap\MARK1 (codon-optimised for Drosophila, 781aa), tagged at the N-terminal with Tag:MYC and at the C-terminal with Tag:HA.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Flies expressing Hsap\MARK1Scer\UAS.T:Hsap\MYC,T:Ivir\HA1 under the control of Scer\GAL4VGlut-OK371 do not show any significant climbing ability defects relative to age-matched controls.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
NOT Enhanced by
Enhancer of
Other
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference

Adult flies co-expressing both Hsap\MARK1Scer\UAS.T:Hsap\MYC,T:Ivir\HA1 and Hsap\MAPTScer\UAS.2N4R.cFa under the control of Scer\GAL4nSyb.PS combined with tub-Gal80[ts] to limit the expression to adulthood show significantly reduced lifespan relative to controls. Similarly, flies simultaneously expressing both these transgenes under the Scer\GAL4VGlut-OK371 driver display age-progressive locomotion deficit (climbing ability defects) that start to be apparent at day 7 post-eclosion.

Hsap\TTBK1Scer\UAS.T:Hsap\MYC,T:Ivir\HA1, Hsap\TTBK2Scer\UAS.T:Hsap\MYC,T:Ivir\HA1, Hsap\MAPTScer\UAS.2N4R.cFa and Hsap\MARK1Scer\UAS.T:Hsap\MYC,T:Ivir\HA1 expressed under the control of Scer\GAL4nSyb.PS combined with tub-Gal80[ts] to limit the expression to adulthood show combinatorial toxicity and lead to short adult lifespan. The short lifespan of adults expressing any two of the above mentioned transgenes is reduced even further when a third one is added (except for simultaneous expression of Hsap\TTBK1Scer\UAS.T:Hsap\MYC,T:Ivir\HA1 and Hsap\TTBK2Scer\UAS.T:Hsap\MYC,T:Ivir\HA1 where the combination with Hsap\MAPTScer\UAS.2N4R.cFa partially alleviates the reduced survival). When Hsap\TTBK1Scer\UAS.T:Hsap\MYC,T:Ivir\HA1, Hsap\TTBK2Scer\UAS.T:Hsap\MYC,T:Ivir\HA1, and Hsap\MARK1Scer\UAS.T:Hsap\MYC,T:Ivir\HA1 are co-expressed, the addition of Hsap\MAPTScer\UAS.2N4R.cFa cannot further shorten the adult lifespan.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Hsap\MARK1Scer\UAS.T:Hsap\MYC,T:Ivir\HA1
Hsap\MARK1UAS.Tag:MYC,Tag:HA
Name Synonyms
Secondary FlyBase IDs
    References (1)