FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Hsap\MAPTUAS.2N4R.cFa
Open Close
General Information
Symbol
Hsap\MAPTUAS.2N4R.cFa
Species
H. sapiens
Name
FlyBase ID
FBal0327423
Feature type
allele
Associated gene
Hsap\MAPT
Associated Insertion(s)
Carried in Construct
PBac{UAS-hMAPT.2N4R.F}
Key Links
Transgenic product class
wild_type
(Fernius et al., 2017)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Fernius et al., 2017)
Mutagen
in vitro construct
(Fernius et al., 2017)
Progenitor genotype
Carried in construct
PBac{UAS-hMAPT.2N4R.F}
Cytology
Description

UAS regulatory sequences drive expression of the Hsap\MAPT 2N4R isoform (2 N terminal domains and 4 R-repeats, 441aa). The ORF was codon-optimised for Drosophila expression and a 'Cavener start-ATG' was added to the 5' end.

(Fernius et al., 2017)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Hsap\MAPT
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  tauopathy
      CEA
      (Vourkou et al., 2022, Fernius et al., 2017)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Hsap\MAPTScer\UAS.2N4R.cFa driven by Scer\GAL4nSyb.PS leads to significantly reduced adult lifespan relative to controls, no significant effect on adult survival is observed when the expression is limited to adulthood by using tub-Gal80[ts].

      Flies expressing Hsap\MAPTScer\UAS.2N4R.cFa under the control of Scer\GAL4VGlut-OK371 show no climbing ability defects early on but they display a slight locomotion deficit at day 14 relative to age-matched controls.

      (Fernius et al., 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Enhancer of
      NOT Enhancer of
      Suppressor of
      Other
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      The reduced adult lifespan characteristic for flies expressing Hsap\TTBK2Scer\UAS.T:Hsap\MYC,T:Ivir\HA1 under the control of Scer\GAL4nSyb.PS combined with tub-Gal80[ts] to limit the expression to adulthood as well as the adult locomotion deficit (climbing ability defects is further decreased by co-expression of Hsap\MAPTScer\UAS.2N4R.cFa.

      Hsap\TTBK1Scer\UAS.T:Hsap\MYC,T:Ivir\HA1, Hsap\TTBK2Scer\UAS.T:Hsap\MYC,T:Ivir\HA1, Hsap\MAPTScer\UAS.2N4R.cFa and Hsap\MARK1Scer\UAS.T:Hsap\MYC,T:Ivir\HA1 expressed under the control of Scer\GAL4nSyb.PS combined with tub-Gal80[ts] to limit the expression to adulthood show combinatorial toxicity and lead to short adult lifespan. The short lifespan of adults expressing any two of the above mentioned transgenes is reduced even further when a third one is added, except for simultaneous expression of Hsap\TTBK1Scer\UAS.T:Hsap\MYC,T:Ivir\HA1 and Hsap\TTBK2Scer\UAS.T:Hsap\MYC,T:Ivir\HA1 where the combination with Hsap\MAPTScer\UAS.2N4R.cFa partially alleviates the reduced survival. When Hsap\TTBK1Scer\UAS.T:Hsap\MYC,T:Ivir\HA1, Hsap\TTBK2Scer\UAS.T:Hsap\MYC,T:Ivir\HA1, and Hsap\MARK1Scer\UAS.T:Hsap\MYC,T:Ivir\HA1 are co-expressed, the addition of Hsap\MAPTScer\UAS.2N4R.cFa cannot further shorten the adult lifespan.

      Adult flies co-expressing both Hsap\MARK1Scer\UAS.T:Hsap\MYC,T:Ivir\HA1 and Hsap\MAPTScer\UAS.2N4R.cFa under the control of Scer\GAL4nSyb.PS combined with tub-Gal80[ts] to limit the expression to adulthood show significantly reduced lifespan relative to controls. Similarly, flies simultaneously expressing both these transgenes under the Scer\GAL4VGlut-OK371 driver display age-progressive locomotion deficit (climbing ability defects) that start to be apparent at day 7 post-eclosion.

      (Fernius et al., 2017)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Hsap\MAPTScer\UAS.2N4R.cFa
      Hsap\MAPTUAS.2N4R.cFa
      hTau2N4R
      (Vourkou et al., 2022)
      Name Synonyms
      Secondary FlyBase IDs
        References (4)