UASp regulatory sequences drive expression of gcl lacking the nuclear localization signal, tagged with 3xTag:FLAG and 3xTag:HA.
Scer\GAL4VP16.nanos.UTR/gclNLS.UASp.Tag:FLAG,Tag:HA rescues gclrev390
The expression of gclNLS.Scer\UAS.P\T.T:Zzzz\FLAG,T:Ivir\HA1 under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 rescues the severe decrease in primordial germ cells in the early embryo (i.e. mitotic cycles 12/13) of gclrev390 homozygotes.