FlyBase Allele Report: Dmel\gcl[rev390]

General Information

Symbol

Dmel\gcl^rev390

Species

D. melanogaster

Name

FlyBase ID

FBal0095410

Feature type

allele

Associated gene

Dmel\gcl

Associated Insertion(s)

Carried in Construct

Also Known As

gcl^Δ

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Faulkner et al., 1998)

Mutagen

Delta2-3 transposase

(Faulkner et al., 1998)

Progenitor genotype

P{lacW}stmA^pngcl

(Faulkner et al., 1998)

Caused by aberration

Df(2R)cmp44E-rev390

Cytology

Description

Imprecise excision of the P{lacW}cmp44E^pngcl element, resulting in a deletion that removes the 3' end of the gcl transcription unit, including the entire gcl coding region.

(Faulkner et al., 1998)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell number | embryonic stage (with gcl^E100K)

(Pae et al., 2017)

decreased cell number | embryonic stage | recessive

(Pae et al., 2017)

female semi-sterile | maternal effect

(Robertson et al., 1999)

viable

(Robertson et al., 1999)

Phenotype Manifest In

germline cell | embryonic stage

(Pae et al., 2017)

pole bud & nucleus

(Leatherman et al., 2002)

pole bud | maternal effect

(Robertson et al., 1999)

primordial germ cell | embryonic stage (with gcl^E100K)

(Pae et al., 2017)

primordial germ cell | maternal effect

(Robertson et al., 1999)

Detailed Description

Statement

Reference

gcl^rev390 homozygotes and gcl^rev390/gcl^E100K transheterozygotes present severe decreases in the number of primordial germ cells in the early embryo (i.e. mitotic cycles 12/13), as compared to wild-type and heterozygous controls; gcl^rev390 heterozygotes do not display any obvious dominant defects in fertility, as shown by the insignificant differences in progression through oogenesis and in laid eggs, as compared to controls.

(Pae et al., 2017)

In gcl^rev390 embryos, the normal transcriptional quiescence seen at nuclear cycle 10 is not seen.

(Leatherman et al., 2002)

When transformed with the cmp44E rescue construct cmp44E^+t8.5, flies homozygous for Df(2R)cmp44E-rev390 lack any visible phenotypes. Mothers homozygous for Df(2R)cmp44E-rev390, with one copy of cmp44E^+t8.5, produce embryos that hatch normally. At the blastoderm stage, however, about half lack any germ cells and the remainder have fewer pole cells than control embryos. At stage 14 about 2/3 of embryos lack pole cells and the pole cell survival rate is much lower than in controls - only about 2/5 of pole cells formed at the blastoderm stage successfully reach the embryonic gonad. The proportion of fertile adults match the proportion of embryos that have at least one pole cell at stage 14. The loss of pole cells appears to occur at Stage 10. Examination of earlier stages reveals that 1/5 embryos fail to form detectable pole buds.

(Robertson et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

gcl^rev390 has decreased cell number | recessive | embryonic stage phenotype, suppressible by tsl⁴/tsl³

(Pae et al., 2017)

gcl^rev390 has decreased cell number | recessive | embryonic stage phenotype, suppressible by tor^HMS00021/Scer\GAL4^{VP16.mat.αTub67C}

(Pae et al., 2017)

gcl^rev390 has decreased cell number | recessive | embryonic stage phenotype, suppressible by tor^GL00222/Scer\GAL4^{VP16.mat.αTub67C}

(Pae et al., 2017)

NOT suppressed by

Statement

Reference

gcl^rev390 has decreased cell number | recessive | embryonic stage phenotype, non-suppressible by tsl[+]/tsl³

(Pae et al., 2017)

gcl^rev390 has decreased cell number | recessive | embryonic stage phenotype, non-suppressible by tsl⁴/tsl[+]

(Pae et al., 2017)

gcl^rev390 has decreased cell number | recessive | embryonic stage phenotype, non-suppressible by Dsor1^GLV21003/Scer\GAL4^{VP16.mat.αTub67C}

(Pae et al., 2017)

gcl^rev390 has decreased cell number | recessive | embryonic stage phenotype, non-suppressible by Dsor1^HMS00145/Scer\GAL4^{VP16.mat.αTub67C}

(Pae et al., 2017)

gcl^rev390 has decreased cell number | recessive | embryonic stage phenotype, non-suppressible by rl^GL00215/Scer\GAL4^{VP16.mat.αTub67C}

(Pae et al., 2017)

gcl^rev390 has decreased cell number | recessive | embryonic stage phenotype, non-suppressible by rl^HMS00173/Scer\GAL4^{VP16.mat.αTub67C}

(Pae et al., 2017)

NOT Suppressor of

Statement

Reference

gcl^rev390/gcl[+] is a non-suppressor of embryonic/larval segmentation phenotype phenotype of tsl⁴/tsl³

(Pae et al., 2017)

gcl^rev390/gcl^rev390 is a non-suppressor of embryonic/larval segmentation phenotype phenotype of tsl⁴/tsl³

(Pae et al., 2017)

Other

Statement

Reference

Cul3[+]/Cul3^gft2, gcl^rev390 has decreased cell number | embryonic stage phenotype

(Pae et al., 2017)

gcl^rev390, tsl⁴/tsl³ has increased cell number | embryonic stage phenotype

(Pae et al., 2017)

Phenotype Manifest In

Suppressed by

Statement

Reference

gcl^rev390 has germline cell | embryonic stage phenotype, suppressible by tsl⁴/tsl³

(Pae et al., 2017)

gcl^rev390 has germline cell | embryonic stage phenotype, suppressible by tor^HMS00021/Scer\GAL4^{VP16.mat.αTub67C}

(Pae et al., 2017)

gcl^rev390 has germline cell | embryonic stage phenotype, suppressible by tor^GL00222/Scer\GAL4^{VP16.mat.αTub67C}

(Pae et al., 2017)

NOT suppressed by

Statement

Reference

gcl^rev390 has germline cell | embryonic stage phenotype, non-suppressible by rl^GL00215/Scer\GAL4^{VP16.mat.αTub67C}

(Pae et al., 2017)

gcl^rev390 has germline cell | embryonic stage phenotype, non-suppressible by rl^HMS00173/Scer\GAL4^{VP16.mat.αTub67C}

(Pae et al., 2017)

gcl^rev390 has germline cell | embryonic stage phenotype, non-suppressible by tsl[+]/tsl³

(Pae et al., 2017)

gcl^rev390 has germline cell | embryonic stage phenotype, non-suppressible by tsl⁴/tsl[+]

(Pae et al., 2017)

gcl^rev390 has germline cell | embryonic stage phenotype, non-suppressible by Dsor1^GLV21003/Scer\GAL4^{VP16.mat.αTub67C}

(Pae et al., 2017)

gcl^rev390 has germline cell | embryonic stage phenotype, non-suppressible by Dsor1^HMS00145/Scer\GAL4^{VP16.mat.αTub67C}

(Pae et al., 2017)

NOT Suppressor of

Statement

Reference

gcl^rev390/gcl[+] is a non-suppressor of embryonic head phenotype of tsl⁴/tsl³

(Pae et al., 2017)

gcl^rev390/gcl[+] is a non-suppressor of embryonic telson phenotype of tsl⁴/tsl³

(Pae et al., 2017)

gcl^rev390/gcl[+] is a non-suppressor of primordial germ cell | embryonic stage phenotype of tsl⁴/tsl³

(Pae et al., 2017)

gcl^rev390/gcl^rev390 is a non-suppressor of embryonic head phenotype of tsl⁴/tsl³

(Pae et al., 2017)

gcl^rev390/gcl^rev390 is a non-suppressor of embryonic telson phenotype of tsl⁴/tsl³

(Pae et al., 2017)

gcl^rev390/gcl^rev390 is a non-suppressor of primordial germ cell | embryonic stage phenotype of tsl⁴/tsl³

(Pae et al., 2017)

Other

Statement

Reference

Cul3[+]/Cul3^gft2, gcl^rev390 has primordial germ cell | embryonic stage phenotype

(Pae et al., 2017)

gcl^rev390, tsl⁴/tsl³ has primordial germ cell | embryonic stage phenotype

(Pae et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

gcl^rev390, Cul3^gft2 double heterozygotes present significant decreases in the number of primordial germ cells in the early embryo (i.e. mitotic cycles 12/13), as compared to wild-type and single heterozygous controls.

The decreased primordial germ cells in gcl^rev390 homozygous early embryos (i.e. mitotic cycles 12/13) is fully suppressed by tsl³/tsl⁴ transheterozygosity or by the Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}-driven expression of tor^HMS00021, tor^GL00222 or tor^GLV21002, but not by tsl³ or tsl⁴ heterozygosity, or by Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}-driven expression of Dsor1^GLV21003, Dsor1^HMS00145, rl^GL00215 or rl^HMS00173. gcl^rev390/+, tsl³/tsl⁴ and gcl^rev390/gcl^rev390, tsl³/tsl⁴ double mutants do not present any obvious defects in either centrosome positioning or division of primordial germ cells in the early embryo, as compared to controls.