Cul3gft2 mutant clones in sensory neurons in adult wing do not display any defects in injury-induced axon degeneration (following an axotomy, the severed axons are cleared away normally).
Cul3gft2 heterozygotes do not display any obvious dominant defects in the number of primordial germ cells in the early embryo (i.e. mitotic cycles 12/13), as compared to controls.
Germline clones of cells homozygous mutant for Cul3gft2 show a ring canal phenotype in the egg chambers: the F-actin cytoskeleton is disorganized, the kel protein accumulates in the lumen and the lumen diameter is significantly smaller compared to controls.
Examination of Cul-3gft2 mutant ring canals in egg chambers reveals a small lumen phenotype and F-actin disorganization.
Homozygous mutant gft2 Mushroom Body clones exhibit axon terminal morphogenetic defects.
Homozygous and gft2/Df(2L)TE35D-15 animals hatch, but die during the second larval instar. Females carrying homozygous germ-line clones produce few eggs and those which are laid are about half the size of wild-type and have fused dorsal appendages. No discernible embryonic structure can be detected interior to the chorion in these eggs. Large homozygous clones in the wing show a number of defects, including defects in the overall shape of the wing and in formation and position of the wing veins. Large clones in the wing often lack trichomes in the centre of the clone. Clones that cover the L3 vein often result in it shifting posteriorly. Clones that cover wing veins are associated with loss of vein tissue. Clones in the wing also result in ectopic sensory organ formation; clones that encompass a large part of the L3 vein have ectopic campaniform sensilla, ectopic campaniform sensilla also arise between wing vein L2 and the anterior wing margin and ectopic bristles arise distally between L2 and L3. Clones in the notum are associated with significant tufting of both micro- and macrochaetae, with the tufts being made up of ectopic fully formed external sensory organs as well as individual sensory organs that contain multiple shafts within a single socket. Homozygous clones in the wing disc show a dramatic increase in the number of sensory organ precursor (SOP) cells, while SOP development outside the clone is normal.
Adult escapers have unexpanded wings and small eyes.
gft2, but not other alleles, fails to