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General Information
Symbol
Dmel\Cul3gft8
Species
D. melanogaster
Name
FlyBase ID
FBal0215312
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

gft8 has a point mutation at the splice acceptor site of the intron between exon 6 and exon 7. This splicing defect may lead to truncation and/or open reading frame shift of gft after amino acid 516, which may eliminate several C-terminal conserved domains, including a Roc1-binding site, Nedd8 modification site, and a 'Cullin' motif.

Nucleotide substitution: G?A.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

neuron & alpha-lobe

neuron & beta-lobe

neuron & gamma-lobe

neuron & mushroom body

Detailed Description
Statement
Reference

The axon terminal defects of gft8 are observed in mutant neuroblast clones, as well as in single-cell/two-cell clones of mutant γ neurons. Mutant neuroblast clones acquire shorter γ and β' lobes than wild-type clones. Most γ processes in the mutant neuroblast clones are only approximately two-thirds the length of wild-type γ processes. In contrast to the truncated γ and β' lobes, only subtle morphological defects are observed in the α, α', and β lobes. These three lobes extend as far as their wild-type counterparts, but their tips are rougher and less dense. Approximately 76% of single-cell/two-cell clones of mutant γ neurons, unlike their wild-type controls, fail to project axons to the tips of the γ lobes.

In wandering larvae, gft8 mutant clones acquire larval mushroom body-specific morphological features, including two perpendicularly oriented lobes, as well as two short bundles of terminal branches. No dramatic morphogenetic defects are observed in gft8 mutant clones at this stage, except subtle deformation of the termini of the medial axon bundles.

By 18 hours after puparium formation, most larval-specific axonal branches are pruned in gft8 mutant clones and look morphologically indistinguishable from their wild-type controls. In contrast to normal pruning, severe regeneration problems in gft8 mutant clones are observed. For instance, by 48 hours after puparium formation, regrowth of the majority of mutant axons is incomplete and appears unsynchronised. No age-dependent morphological changes are detected in gft8 clones after eclosion.

gft8 mutant γ axon clones exhibit few arbors. Approximately 57% of fully extended mutant γ neurons have no major (defined as over 15υm) axon arbors (compared to 15% in wild-type), suggesting that arborization is suppressed in mutant γ axons. However, a similar percentage of γ neurons (14% in mutant, 15% in wild-type) acquire fully extended 'arbor-free' axons, regardless of the size of arbor.

gft8 single cell mutant γ processes can terminate within any part of the γ lobes.

gft8 single cell mutant neuroblast clones exhibit a subtle phenotype in the α lobes.

Truncation occurs in 25% of gft8 β processes. All truncated β axons stall within either the proximal one-third segments or the distal one-third segments of the β lobes, close to the positions at which two clusters of arbors form in wild-type β processes.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of gftWT.T:Avic\GFP.Scer\UAS under the control of Scer\GAL4OK107 in gft8 mutant clones rescues the axon terminal morphogenesis phenotype.

Expression of gftWT.T:Avic\GFP.Scer\UAS under the control of Scer\GAL4OK107 in gft8 mutant clones fails to rescue the axon terminal morphogenesis phenotype.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (7)
Reported As
Symbol Synonym
Cul-3gft8
Cul3gft8
cul-38
cul-3gft8
gft8
l(2L)MB397
Name Synonyms
Secondary FlyBase IDs
    References (2)