FlyBase Allele Report: Dmel\tsl[4]

General Information

Symbol

Dmel\tsl⁴

Species

D. melanogaster

Name

FlyBase ID

FBal0017198

Feature type

allele

Associated gene

Dmel\tsl

Associated Insertion(s)

Carried in Construct

Also Known As

tsl⁶⁹¹

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Savant-Bhonsale and Montell, 1993, Tearle and Nusslein-Volhard, 1987)

Progenitor genotype

Cytology

Description

Amino acid replacement: A263V.

(Savant-Bhonsale and Montell, 1993)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:21,778,626..21,778,626 [-]

Nucleotide change:

C21778626T

Amino acid change:

A263V | tsl-PA; A263V | tsl-PB; A263V | tsl-PC

Reported amino acid change:

A263V

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Savant-Bhonsale and Montell, 1993)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

abdominal segment 8 | embryonic stage | maternal effect (with tsl^Δ)

(Johnson et al., 2017)

embryonic/larval cuticle | embryonic stage | maternal effect (with tsl^Δ)

(Johnson et al., 2017)

embryonic head (with tsl³)

(Pae et al., 2017)

embryonic head | maternal effect (with tsl^Δ)

(Johnson et al., 2017)

embryonic telson (with tsl³)

(Pae et al., 2017)

filzkorper

(Cinnamon et al., 2004)

filzkorper | embryonic stage | maternal effect (with tsl^Δ)

(Johnson et al., 2017)

primordial germ cell | embryonic stage (with tsl³)

(Pae et al., 2017)

telson | embryonic stage | maternal effect (with tsl^Δ)

(Johnson et al., 2017)

Detailed Description

Statement

Reference

Late embryos from tsl⁴/tsl^Δ transheterozygous mothers exhibit a fully penetrant "terminal class mutant" phenotype, in which some terminal structures are missing (including head structures, abdominal segment 8, telson, and filzkorper), and exhibit a highly penetrant posterior-ventral cuticle hole phenotype, as compared to controls.

(Johnson et al., 2017)

tsl⁴/tsl³ transheterozygotes present a pole-hole phenotype during early embryonic stage and present patterning defects at late embryonic stage, as shown by head and telson defects in cuticle preparations, as compared to controls.

(Pae et al., 2017)

Pupariation is delayed in tsl⁴/Df(3R)caki^X-313 mutant larvae.

Pupariation is delayed in homozygous tsl⁴ mutant larvae.

Pupariation is delayed in tsl⁴/tsl^X-307 mutant larvae.

(Grillo et al., 2012)

Embryos derived from tor^12D/+ ; tsl⁴/tsl⁴ females have the same phenotype as embryos derived from tor^12D/+ females, indicating that tsl functions upstream of tor.

(Stevens et al., 1990)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

tsl⁴/tsl³ has embryonic/larval segmentation phenotype phenotype, non-suppressible by gcl^rev390/gcl[+]

(Pae et al., 2017)

tsl⁴/tsl³ has embryonic/larval segmentation phenotype phenotype, non-suppressible by gcl^rev390/gcl^rev390

(Pae et al., 2017)

Suppressor of

Statement

Reference

tsl⁴/tsl³ is a suppressor of decreased cell number | recessive | embryonic stage phenotype of gcl^rev390

(Pae et al., 2017)

NOT Suppressor of

Statement

Reference

tsl⁴/tsl[+] is a non-suppressor of decreased cell number | recessive | embryonic stage phenotype of gcl^rev390

(Pae et al., 2017)

Other

Statement

Reference

gcl^rev390, tsl⁴/tsl³ has increased cell number | embryonic stage phenotype

(Pae et al., 2017)

Phenotype Manifest In

Suppressed by

Statement

Reference

tsl⁴ has filzkorper phenotype, suppressible by Dp(3;3)bicS

(Cinnamon et al., 2004)

tsl⁴ has embryo | anterior phenotype, suppressible by bcd^+t8.7

(Schaeffer et al., 2000)

tsl⁴ has embryo | anterior phenotype, suppressible by bcd^+t8

(Schaeffer et al., 2000)

NOT suppressed by

Statement

Reference

tsl⁴/tsl³ has embryonic head phenotype, non-suppressible by gcl^rev390/gcl[+]

(Pae et al., 2017)

tsl⁴/tsl³ has embryonic telson phenotype, non-suppressible by gcl^rev390/gcl[+]

(Pae et al., 2017)

tsl⁴/tsl³ has primordial germ cell | embryonic stage phenotype, non-suppressible by gcl^rev390/gcl[+]

(Pae et al., 2017)

tsl⁴/tsl³ has embryonic head phenotype, non-suppressible by gcl^rev390/gcl^rev390

(Pae et al., 2017)

tsl⁴/tsl³ has embryonic telson phenotype, non-suppressible by gcl^rev390/gcl^rev390

(Pae et al., 2017)

tsl⁴/tsl³ has primordial germ cell | embryonic stage phenotype, non-suppressible by gcl^rev390/gcl^rev390

(Pae et al., 2017)

Suppressor of

Statement

Reference

tsl⁴/tsl³ is a suppressor of germline cell | embryonic stage phenotype of gcl^rev390

(Pae et al., 2017)

NOT Suppressor of

Statement

Reference

tsl⁴/tsl[+] is a non-suppressor of germline cell | embryonic stage phenotype of gcl^rev390

(Pae et al., 2017)

Other

Statement

Reference

gcl^rev390, tsl⁴/tsl³ has primordial germ cell | embryonic stage phenotype

(Pae et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The pole-hole phenotype in early embryonic stage and the patterning defects in late embryonic stage (i.e. head and telson defects in cuticle preparations) displayed by tsl⁴/tsl³ transheterozygotes are not suppressed by gcl^rev390 heterozygosity or homozygosity.

The decreased primordial germ cells in gcl^rev390 homozygous early embryos (i.e. mitotic cycles 12/13) is fully suppressed by tsl⁴/tsl³ transheterozygosity, but not by tsl⁴ heterozygosity. tsl⁴/tsl³, gcl^rev390/+ and tsl⁴/tsl³, gcl^rev390/gcl^rev390 double mutants do not present any obvious defects in either centrosome positioning or cell division of primordial germ cells in the early embryo, as compared to controls.

(Pae et al., 2017)

Four copies of the bcd gene (bcd^+t8.7 or bcd^+t8) are able to rescue some anterior structures in about 40% of tsl⁴ derived embryos. However not all embryos with rescued labrum and dorsal bridge have a perfectly aligned head skeleton. Occasionally embryos survive long enough to hatch and move around.

(Schaeffer et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (6)

Bloomington

3289

e¹ tsl⁴/TM1

3259

st¹ kni^ri-1 rn^roe-1 p^p osk⁶ e¹ tsl⁴ ca¹/TM3, Sb¹

95110

w^*; PBac{αTub67C-bcd-LEXY(FRT.bcd3'UTR)sqh3'UTR}VK00033 bcd⁶ nanos^BN e¹ tsl⁴/TM3, Sb¹

95109

w^*; PBac{αTub67C-iRFP713-bcd-LEXY(FRT.bcd3'UTR)sqh3'UTR}VK00033 bcd⁶ nanos^BN e¹ tsl⁴/TM3, Sb¹

Kyoto

106960

e¹ tsl⁴/TM1

106985

kni⁹ tsl⁴ e¹/TM6C, Sb¹ Tb¹

Notes on Origin

Discoverer

Comments

Clonal analysis indicates that tsl expression is required only in subpopulations of follicle cells located at the poles of the oocyte.

(Stevens et al., 1990)

Allelic series: tsl⁵ = tsl⁴ > tsl³ > tsl¹ > tsl²

(Savant-Bhonsale and Montell, 1993)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

torso-like⁴

(Wang et al., 2013)

torsolike⁴

(Rahimi et al., 2016)

tsl4

(Grillo et al., 2012)

tsl⁴

(Ho et al., 2025, Ho et al., 2023, Singh et al., 2022, Hannon et al., 2017, Johnson et al., 2017, Pae et al., 2017)

tsl⁶⁹¹

(Johnson et al., 2020, Helman et al., 2011, Cinnamon et al., 2008, Cinnamon et al., 2004, Van Doren et al., 1998, Gabay et al., 1997, Greenwood and Struhl, 1997, Paroush et al., 1997, Schulz and Tautz, 1995, Klingler and Gergen, 1993, Savant-Bhonsale and Montell, 1993, Sprenger and Nusslein-Volhard, 1993, Stevens et al., 1990, Tearle and Nusslein-Volhard, 1987)

tsl^A263V

(Pae et al., 2017)

Name Synonyms

Secondary FlyBase IDs

References (27)

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