FlyBase Allele Report: Hsap\RELA[AD.R12G04]

General Information

Symbol

Hsap\RELA^AD.R12G04

Species

H. sapiens

Name

FlyBase ID

FBal0328346

Feature type

allele

Associated gene

Hsap\RELA

Associated Insertion(s)

Carried in Construct

P{R12G04-p65.AD}

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Rubin Laboratory at Janelia Farm, 2017)

Progenitor genotype

Carried in construct

P{R12G04-p65.AD}

Cytology

Description

The GMR12G04 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain).

(Rubin Laboratory at Janelia Farm, 2017)

Allele components

Component

Use(s)

Regulatory region(s)

GMR12G04

Encoded product / tool

p65(AD)::Zip+

split driver - transcription activation fragment

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

LNd neuron | subset

Comment: when combined with P{R18D09-GAL4.DBD} (combination referred to as 'MB122B')

(Liang et al., 2017)

adult Pdf negative s-LNv neuron

Comment: when combined with P{R18D09-GAL4.DBD} (combination referred to as 'MB122B')

(Liang et al., 2017)

adult lateral accessory lobe-anterior nodulus 3 neuron

Comment: when combined with P{VT046049-GAL4.DBD} (combination referred to as 'SS47436')

(Wolff and Rubin, 2018)

adult neuron | subset of adult abdominal neuromere

Comment: when combined with P{R73H04-GAL4.DBD} (combination referred to as 'SS39947')

(Sterne et al., 2021)

adult whisker subesophageal neuron

Comment: when combined with P{R73H04-GAL4.DBD} (combination referred to as 'SS39947')

(Sterne et al., 2021)

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell number | adult stage, with Scer\GAL4^DBD.R18D09, Spyo\Cas9^UAS.P2, per^{UAS.gRNAx4.pCFD6}

(Saurabh et al., 2024)

abnormal circadian behavior | adult stage, with Scer\GAL4^DBD.R18D09, Spyo\Cas9^UAS.P2, per^{UAS.gRNAx4.pCFD6}

(Saurabh et al., 2024)

abnormal circadian behavior | adult stage, with Scer\GAL4^DBD.R18D09, Spyo\Cas9^UAS.P2, tim^{UAS.gRNAx3.pCFD6}

(Saurabh et al., 2024)

abnormal feeding behavior | adult stage, with Scer\GAL4^DBD.R18D09, Spyo\Cas9^UAS.P2, per^{UAS.gRNAx4.pCFD6}

(Saurabh et al., 2024)

abnormal feeding behavior | adult stage, with Scer\GAL4^DBD.R18D09, Spyo\Cas9^UAS.P2, tim^{UAS.gRNAx3.pCFD6}

(Saurabh et al., 2024)

abnormal locomotor rhythm | adult stage, with Hsap\KCNJ2^UAS.EGFP, Scer\GAL4^DBD.R18D09

(Bulthuis et al., 2019)

abnormal locomotor rhythm | adult stage, with Scer\GAL4^DBD.R18D09, kcc^KK107965

(Eick et al., 2022)

decreased rate of movement | adult stage, with Scer\GAL4^DBD.R18D09, Spyo\Cas9^UAS.P2, tim^{UAS.gRNAx3.pCFD6}

(Saurabh et al., 2024)

Phenotype Manifest In

adult brain, with Scer\GAL4^DBD.R18D09, Spyo\Cas9^UAS.P2, per^{UAS.gRNAx4.pCFD6}

(Saurabh et al., 2024)

Detailed Description

Statement

Reference

Elimination of molecular clock function (using cyc^{Δ.UAS.Tag:MYC}) in neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R18D09 and Hsap\RELA^AD.R12G04 in adults does not affect the strength of the circadian rhythm of sleep-wake cycles, compared to controls.

Inhibition (using Hsap\KCNJ2^UAS.EGFP) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R18D09 and Hsap\RELA^AD.R12G04 significantly reduces the strength of the circadian rhythm of sleep-wake cycles, compared to controls; this is also the case when inhibition is restricted to the adult stage using a Gal80[ts] transgene.

(Bulthuis et al., 2019)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Hsap\KCNJ2^{10xUAS.EGFP.FLP-OFF}, Hsap\RELA^AD.R12G04, Scer\GAL4^DBD.R18D09 has abnormal feeding behavior | adult stage phenotype

(Saurabh et al., 2024)

Hsap\KCNJ2^{10xUAS.EGFP.FLP-OFF}, Hsap\RELA^AD.R12G04, Scer\GAL4^DBD.R18D09 has decreased rate of movement | adult stage phenotype