FlyBase Allele Report: Hsap\RELA[AD.R26D04]

General Information

Symbol

Hsap\RELA^AD.R26D04

Species

H. sapiens

Name

FlyBase ID

FBal0328629

Feature type

allele

Associated gene

Hsap\RELA

Associated Insertion(s)

Carried in Construct

P{R26D04-p65.AD}

PBac{R26D04-p65.AD}

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Rubin Laboratory at Janelia Farm, 2017)

Progenitor genotype

Carried in construct

P{R26D04-p65.AD}

PBac{R26D04-p65.AD}

Cytology

Description

The GMR26D04 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain).

(Rubin Laboratory at Janelia Farm, 2017)

Allele components

Component

Use(s)

Regulatory region(s)

GMR26D04

Encoded product / tool

p65(AD)::Zip+

split driver - transcription activation fragment

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

larval A03a5 neuron

Comment: when combined with P{R48H02-GAL4.DBD} (combination referred to as 'SS02064')

(Takagi et al., 2017)

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | larval stage | heat sensitive, with Scer\GAL4^DBD.R48H02, shi^1.UAS

(Takagi et al., 2017)

abnormal touch response | larval stage | heat sensitive, with Scer\GAL4^DBD.R48H02, shi^1.UAS

(Takagi et al., 2017)

increased rate of movement | larval stage | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Scer\GAL4^DBD.R48H02

(Takagi et al., 2017)

Phenotype Manifest In

embryonic/larval hypodermal muscle cell | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Scer\GAL4^DBD.R48H02

(Takagi et al., 2017)

Detailed Description

Statement

Reference

Optogenetic activation (using Cnoc\ChR1^{Cs.IVS.20xUAS.Venus} with 660 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R48H02 and Hsap\RELA^AD.R26D04 in larvae induces musculature contractions that result in tail flicking.

Inhibition (using shi^1.UAS at 31[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R48H02 and Hsap\RELA^AD.R26D04 in larvae significantly reduces the probability of backwards motion in response to a head touch, with no effect on the peristaltic motion itself, compared to controls.

(Takagi et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions