FlyBase Allele Report: Hsap\RELA[AD.R82E12]

General Information

Symbol

Hsap\RELA^AD.R82E12

Species

H. sapiens

Name

FlyBase ID

FBal0329342

Feature type

allele

Associated gene

Hsap\RELA

Associated Insertion(s)

Carried in Construct

P{R82E12-p65.AD}

Also Known As

82E12-Gal4^AD

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Rubin Laboratory at Janelia Farm, 2017)

Progenitor genotype

Carried in construct

P{R82E12-p65.AD}

Cytology

Description

The GMR82E12 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain).

(Rubin Laboratory at Janelia Farm, 2017)

Allele components

Component

Use(s)

Regulatory region(s)

GMR82E12

Encoded product / tool

p65(AD)::Zip+

split driver - transcription activation fragment

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

larval A08n neuron

Comment: when combined with P{Rab11-GAL4.DBD.6.14.3}

(Hu et al., 2017)

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | larval stage | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Scer\GAL4^{DBD.Rab11.6.14.3}

(Hu et al., 2017)

abnormal pain response | larval stage, with Ctet\tetX^TNT-LC.UAS, Scer\GAL4^{DBD.Rab11.6.14.3}

(Hu et al., 2017)

abnormal pain response | larval stage, with Hsap\KCNJ2^UAS.EGFP, Scer\GAL4^{DBD.Rab11.6.14.3}

(Hu et al., 2017)

Phenotype Manifest In

Detailed Description

Statement

Reference

Hyperpolarization (using Hsap\KCNJ2^UAS.EGFP) of neurons targeted by the split-GAL4 pair of Scer\GAL4^{DBD.Rab11.6.14.3} and Hsap\RELA^AD.R82E12 in larvae significantly reduces nociceptive movement responses following stimulation with a von Frey filament, compared to controls; there is no effect on the response to gentle touch or locomotion speed, compared to controls.

Inhibition (using Ctet\tetX^TNT-LC.UAS) of neurons targeted by the split-GAL4 pair of Scer\GAL4^{DBD.Rab11.6.14.3} and Hsap\RELA^AD.R82E12 in larvae significantly reduces nociceptive movement responses following stimulation with a von Frey filament, but does not affect the latency of rolling in response to high temperature (local) stimulation, compared to controls.

Optogenetic activation (using Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus} with 625 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4^{DBD.Rab11.6.14.3} and Hsap\RELA^AD.R82E12 in larvae significantly increases rolling responses in the absence of pain stimuli, compared to controls.

(Hu et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions