FB2021_02, released April 13, 2021

Allele: Scer\GAL4DBD.R86D05

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General Information
Symbol
Scer\GAL4DBD.R86D05
Species
S. cerevisiae
Name
FlyBase ID
FBal0332538
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
Carried in Construct
P{R86D05-GAL4.DBD}
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
in vitro construct - regulatory fusion
(Rubin Laboratory at Janelia Farm, 2017)
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
       
      Progenitor genotype
      Carried in construct
      P{R86D05-GAL4.DBD}
      Cytology
      Nature of the lesion
      Statement
      Reference

      The GMR86D05 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain).

      (Rubin Laboratory at Janelia Farm, 2017)
      Allele components
      Product class / Tool use(s)
      Encoded product / tool
      GAL4(DBD)::Zip-
      Associated Sequence Features
      GMR86D05
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      lobula columnar neuron LC4

      Comment: when combined with P{R47H03-p65.AD} (combination referred to as 'SS00356')

      (von Reyn et al., 2017)
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Optogenetic activation (using Cnoc\ChR1Cs.IVS.20xUAS.Venus with 590 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R86D05 and Hsap\RELAAD.R47H03 in adults significantly increases takeoff rates, compared to controls; takeoffs are shorter in duration than spontaneous takeoffs in controls.

      Silencing (using Hsap\KCNJ2IVS.10xUAS.EGFP) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R86D05 and Hsap\RELAAD.R47H03 in adults significantly reduces overall takeoff rates and the proportion of short takeoffs in response to fast, but not slow, looming stimuli, compared to controls.

      (von Reyn et al., 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Scer\GAL4DBD.R86D05.T:Zzzz\Zip-
      Scer\GAL4DBD.R86D05
      Name Synonyms
      Secondary FlyBase IDs
        References (3)