FB2026_02 , released June 18, 2026
Allele: Dmel\CaMKIIstopw+
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General Information
Symbol
Dmel\CaMKIIstopw+
Species
D. melanogaster
Name
FlyBase ID
FBal0337891
Feature type
allele
Associated gene
Dmel\CaMKII
Associated Insertion(s)
TI{TI}CaMKIIstopw+
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ends-out gene targeting
(Kuklin et al., 2017)
Progenitor genotype
Associated Insertion(s)
TI{TI}CaMKIIstopw+
Cytology
Description

Two in-frame stop codons have been introduced into exon 2, at the beginning of the CaMKII coding region (the normal translation start codon has been mutated to TAG and an additional stop has been added at codon 7). A loxP cassette containing a w+* marker is also present (in the intron between exons 4 and 5).

(Kuklin et al., 2017)
Allele components
Component
Use(s)
Also carries
loxP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      CaMKIIstopw+ homozygotes are lethal and the lethal phase spans both larval and pupal stage. Both the brain neuropil and the neuromuscular junction (NMJ) morphology in CaMKIIstopw+ mutant third instar larvae do not display any obvious defects, the number of NMJ boutons or the postsynaptic area are both comparable to controls. Neuronal transmission at the NMJ also appears largely intact: the amplitude and dynamics of excitatory junction potentials (EJP), the facilitation/depression index, amplitude of spontaneous activity as well as quantal content are all unaffected, only the frequency of miniature EJPs is significantly reduced compared to wild-type sibling controls, although the density of active zones (marked by brp) is not changed. CaMKIIstopw+ mutant larvae display a slight decreased in the area of muscles 6+7 and their muscles appear more fragile.

      (Kuklin et al., 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      CaMKIIstopw+ is rescued by CaMKIIFlyFos025846

      (Kuklin et al., 2017)
      Partially rescued by

      CaMKIIstopw+ is partially rescued by CaMKIIΔUTR.FlyFos025846

      (Kuklin et al., 2017)
      Comments

      Combination with CaMKIIFlyFos025846 rescues the lethality of CaMKIIstopw+ homozygotes and the spontaneous activity at the neuromuscular junction of the rescue larvae (rate of miniature excitatory junction potentials, mEJPs) is comparable to wild-type controls, whereas combination with CaMKIIΔUTR.FlyFos025846 only partially rescues the lethality of CaMKIIstopw+ mutants and is also unable to fully rescue the reduced frequency of miniature excitatory junction potentials at larval neuromuscular junctions.

      (Kuklin et al., 2017)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      CaMKIIstopw+
      (Kuklin et al., 2017)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)