FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\RhoGEF3KAA.UAS.sfGFP
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General Information
Symbol
Dmel\RhoGEF3KAA.UAS.sfGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0338000
Feature type
allele
Associated gene
Dmel\RhoGEF3
Associated Insertion(s)
Carried in Construct
PBac{UAS-RhoGEF3.KAA.sfGFP}
Key Links
Transgenic product class
missense_variant
(Couturier et al., 2017)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Couturier et al., 2017)
Mutagen
in vitro construct
(Couturier et al., 2017)
Progenitor genotype
Carried in construct
PBac{UAS-RhoGEF3.KAA.sfGFP}
Cytology
Description

UAS regulatory sequences drive expression of RhoGEF3 in which the E, K and N residues required for GEF activity have been mutated to K, A and A respectively. The protein is tagged at the C-terminal end (which is shared by all predicted isoforms) with a sfGFP tag flanked by GVG linkers.

(Couturier et al., 2017)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
RhoGEF3
Tagged with
sfGFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The expression of RhoGEF3KAA.UAS.sfGFP under the control of Scer\GAL4ap.PU and Gal80[ts] has no effect on F-actin distribution and epithelium shape in the dorsal cells of wing imaginal discs, as compared to controls.

      The expression of RhoGEF3KAA.UAS.sfGFP under the control of Scer\GAL4GMR.PU at 25[o]C leads to no obvious visible effects on the adult eye, as compared to controls.

      (Couturier et al., 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhancer of
      Statement
      Reference

      RhoGEF3KAA.UAS.sfGFP/Scer\GAL4GMR.PU is an enhancer of eye | adult stage phenotype of Rac1UAS.cWa

      (Couturier et al., 2017)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The expression of RhoGEF3KAA.UAS.sfGFP in combination with Rac1Scer\UAS.cWa under the control of Scer\GAL4GMR.PU leads to a moderate decrease in adult eye size, which is more severe than upon the Scer\GAL4GMR.PU-driven expression of Rac1Scer\UAS.cWa alone. Expression of RhoGEF3UAS.EKN.sfGFP in combination with either Cdc42Scer\UAS.cWa or Rho1Scer\UAS.P\T.T:Uuuu\T7 under the control of Scer\GAL4GMR.PU leads to no obvious visible effects on the adult eye, as compared to controls.

      (Couturier et al., 2017)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      RhoGEF3KAA.Scer\UAS.T:Avic\GFP-SF
      RhoGEF3KAA.UAS.sfGFP
      Name Synonyms
      Secondary FlyBase IDs
        References (1)