FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Rac1UAS.cWa
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General Information
Symbol
Dmel\Rac1UAS.cWa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Warner
FlyBase ID
FBal0246545
Feature type
allele
Associated gene
Dmel\Rac1
Associated Insertion(s)
Carried in Construct
P{UAS-Rac1.W}
Also Known As
UAS-rac1
Key Links
Transgenic product class
wild_type
(Warner, 2010.9.15)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Warner, 2010.9.15)
Mutagen
in vitro construct
(Warner, 2010.9.15)
Progenitor genotype
Carried in construct
P{UAS-Rac1.W}
Cytology
Description

UAS regulatory sequences drive expression of wild-type Rac1.

(Warner, 2010.9.15)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Rac1
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The expression of Rac1Scer\UAS.cWa under the control of Scer\GAL4GMR.PU at 18[o]C leads to a relatively minor decrease in adult eye size, as compared to controls.

      (Couturier et al., 2017)

      Ectopic expression of Rac1Scer\UAS.cWa under the control of Scer\GAL4GMR.PFa does not disrupt patterning of the ommatidial precursor clusters in the third instar larval eye disc compared to controls. Expression under the control of the Scer\GAL4GMR.long driver leads to 'rough eye' phenotype in adults.

      (Ruan et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Enhancer of
      Suppressor of
      Other
      Phenotype Manifest In
      Enhanced by
      Statement
      Reference

      Rac1UAS.cWa has eye | adult stage phenotype, enhanceable by RhoGEF3KAA.UAS.sfGFP/Scer\GAL4GMR.PU

      (Couturier et al., 2017)
      Enhancer of
      Suppressor of
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The expression of Rac1Scer\UAS.cWa in combination with RhoGEF3UAS.EKN.sfGFP, but not RhoGEF3KAA.UAS.sfGFP, under the control of Scer\GAL4GMR.PU leads to a fully penetrant pharate lethality. Co-expression with RhoGEF3UAS.EKN.sfGFP is associated with a very strong eye defect (residual eye) as compared to controls, whereas co-expression with RhoGEF3KAA.UAS.sfGFP leads to a moderate decrease in adult eye size, which is more severe than upon the Scer\GAL4GMR.PU-driven expression of Rac1Scer\UAS.cWa alone.

      (Couturier et al., 2017)

      Co-expression of egrScer\UAS.cMa and Rac1Scer\UAS.cWa under the control of Scer\GAL4GMR.PFa leads to pupal stage lethality and perturbed patterning of the third instar larval eye disc is observed: the regular spacing of ommatidial precursor clusters (visualized by Chaoptin) is completely disrupted with some R-cells shifted to the optic stalk. Co-expression under the control of the Scer\GAL4GMR.long driver allows survival to adult stage but the malformed adult eye phenotype observed in flies expressing either egrScer\UAS.cMa (small 'rough eye') or Rac1Scer\UAS.cWa ('rough eye') alone is strongly enhanced, resulting in a 'no eye' phenotype.

      (Ruan et al., 2016)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Rac1Scer\UAS.cWa
      Rac1UAS.cWa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Warner
      Secondary FlyBase IDs
        References (6)