The potential native promoter of pnut (1.7kb upstream of the translation start site) drives expression of a wild-type pnut cDNA. The open reading frame is tagged at the N-terminal end with Tag:FLAG.
pnutWT.Tag:FLAG rescues pnutmut1
Combination with pnutWT.T:Zzzz\FLAG rescues the larval lethality of pnutmut1 homozygous mutants and the emerging adult females are fertile.