FB2026_02 , released June 18, 2026
Allele: Dmel\Graf1
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General Information
Symbol
Dmel\Graf1
Species
D. melanogaster
Name
FlyBase ID
FBal0338008
Feature type
allele
Associated gene
Dmel\Graf
Associated Insertion(s)
Carried in Construct
Key Links
Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Kim et al., 2017)
Mutagen
P-element activity
(Kim et al., 2017)
Progenitor genotype
P{EP}GrafG275
(Kim et al., 2017)
Cytology
Description

2153bp deletion that removes the entire first exon and part of the first intron of Graf. Generated by imprecise excision of P{EP}GrafG275.

(Kim et al., 2017)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      model of  Billuart-type X-linked syndromic intellectual developmental disorder
      is ameliorated by Egfrf24
      (Kim et al., 2021)
      is ameliorated by Hsap\OPHN1UAS.Tag:HA
      (Kim et al., 2021)
      is ameliorated by rl10
      (Kim et al., 2021)
      is ameliorated by rl1
      (Kim et al., 2021)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      In the Graf1/Df(1)BSC756 adult brain, mushroom body Β-axon fibers overextend beyond the midline, such that the Β lobes from both hemispheres appear to fuse; this phenotype starts being seen during the pupal stage, from 36h APF onward. Graf1 hemizygous adult males show a similar phenotype; in addition they exhibit a significant decrease in long-term memory (in aversive olfactory learning assays).

      Graf1 homozygous mushroom body Β cell clones overextend beyond the midline.

      (Kim et al., 2021)

      Homozygous Graf1 mutants are viable and fertile.

      Graf1/Df(1)BSC756 or Graf1/Y mutant third instar larvae show much higher levels of circulating and total (i.e. circulating plus sessile) hemocytes and also have significantly more hemocytes in the primary lymph gland lobe. This is attributable to increased production of plasmatocytes as the levels of neither circulating or sessile crystal cells nor the percentage of lamellocytes is changed relative to controls and it is also just the plasmatocyte population that is selectively increased in the lymph gland. This plasmatocyte overabundance appears to be primarily due to increased proliferation as the number of mitotically active hemocytes is significantly increased in both second and third instar larvae.

      Primary hemocytes from Graf1/Df(1)BSC756 larvae show fluid-phase uptake deficiency and strongly reduced internalization of sSpi-GFP protein (spi encodes the principal ligand of the EGFR pathway) at high sSpi-GFP conditions (treatment with 30 ng/ml sSpi-GFP) but not at low sSpi-GFP conditions (treatment with 4 ng/ml sSpi-GFP), as compared to controls.

      (Kim et al., 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressed by
      Statement
      Reference

      Df(1)BSC756/Graf1 has adult mushroom body beta-lobe phenotype, suppressible | partially by Scer\GAL4ey-OK107/Hsap\OPHN1UAS.Tag:HA

      (Kim et al., 2021)

      Df(1)BSC756/Graf1 has axon | adult stage phenotype, suppressible | partially by Scer\GAL4ey-OK107/Hsap\OPHN1UAS.Tag:HA

      (Kim et al., 2021)

      Df(1)BSC756/Graf1 has midline | adult stage phenotype, suppressible | partially by Scer\GAL4ey-OK107/Hsap\OPHN1UAS.Tag:HA

      (Kim et al., 2021)

      Graf1 has adult mushroom body beta-lobe phenotype, suppressible by Egfr[+]/Egfrf24

      (Kim et al., 2021)

      Graf1 has axon | adult stage phenotype, suppressible by Egfr[+]/Egfrf24

      (Kim et al., 2021)

      Graf1 has midline | adult stage phenotype, suppressible by Egfr[+]/Egfrf24

      (Kim et al., 2021)

      Graf1 has axon | adult stage phenotype, suppressible by rl1/rl10

      (Kim et al., 2021)

      Graf1 has adult mushroom body beta-lobe phenotype, suppressible by rl1/rl10

      (Kim et al., 2021)

      Graf1 has midline | adult stage phenotype, suppressible by rl1/rl10

      (Kim et al., 2021)

      Graf1 has axon | adult stage phenotype, suppressible | partially by rl[+]/rl1

      (Kim et al., 2021)

      Graf1 has adult mushroom body beta-lobe phenotype, suppressible | partially by rl[+]/rl1

      (Kim et al., 2021)

      Graf1 has midline | adult stage phenotype, suppressible | partially by rl[+]/rl1

      (Kim et al., 2021)

      Graf1 has hemocyte | increased number | third instar larval stage phenotype, suppressible by Egfr[+]/Egfrf24

      (Kim et al., 2017)

      Graf1 has hemocyte | increased number | third instar larval stage phenotype, suppressible by Ras85De2F/Ras85D[+]

      (Kim et al., 2017)

      Graf1 has hemocyte | increased number | third instar larval stage phenotype, suppressible | partially by rl[+]/rl1

      (Kim et al., 2017)

      Graf1 has hemocyte | increased number | third instar larval stage phenotype, suppressible by rl1/rl10

      (Kim et al., 2017)

      Graf1 has hemocyte | increased number | third instar larval stage phenotype, suppressible by spi1/spi[+]

      (Kim et al., 2017)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The increased concentration of circulating hemocytes characteristic for Graf1/Y mutant third instar larvae is suppressible by combination with a single copy of Egfrf24, Ras85De2F or spi1 and partially so with rl1; combination with rl1/rl10 reduces the hemocyte levels in Graf1/Y larvae to below wild-type levels, comparable to those seen in rl1/rl10 single mutants.

      (Kim et al., 2017)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      Df(1)BSC756/Graf1 is rescued by Scer\GAL4crq.PO/GrafUAS.Tag:HA

      (Kim et al., 2017)
      Partially rescued by

      Graf1 is partially rescued by Scer\GAL4ey-OK107/GrafUAS.Tag:HA

      (Kim et al., 2021)

      Df(1)BSC756/Graf1 is partially rescued by Scer\GAL4elav-C155/GrafUAS.Tag:HA

      (Kim et al., 2021)

      Df(1)BSC756/Graf1 is partially rescued by Scer\GAL4ey-OK107/GrafUAS.Tag:HA

      (Kim et al., 2021)
      Comments

      The increased number of total hemocytes (i.e. circulating plus sessile) observed in Graf1/Df(1)BSC756 mutant third instar larvae is fully rescued by expression of GrafScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4crq.PO in the mutant background. Scer\GAL4crq.PO-driven expression of GrafScer\UAS.T:Ivir\HA1 also rescues the fluid-phase uptake deficiency characteristic for primary hemocytes isolated from Graf1/Df(1)BSC756 larvae.

      (Kim et al., 2017)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Graf1
      (Kim et al., 2021, Kim et al., 2017)
      Name Synonyms
      Secondary FlyBase IDs
        References (2)