In the third instar larval stage, ScerGAL4SIFaR.PS drives expression in a bilateral pair of neurons per larval abdominal neuromere. In adults, it drives expression in all brain areas that are innervated by SIFa positive neurons and , in females, two neurons of the uterus that extend into the central nervous system. In the brain, some peptidergic neurons were individually positive for FMRFa or AstA.
Ablation (using rprUAS.C) of Scer\GAL4SIFaR.PS cells significantly increases male-male courtship behavior, compared to controls.