amorphic allele - molecular evidence

A TI{CRIMIC.TG4.0} DNA cassette has been inserted into CG5022, in a coding intron, and is predicted to gene trap all annotated transcripts of the gene. The TI{CRIMIC.TG4.0} cassette was inserted using the CRISPR/Cas9 technique together with a donor plasmid to drive homology directed repair. The sgRNA sequence used to target the gene was: TTCGGCTGTAAACAGATGGGTGG. The homology arms of the donor plasmid used were designed such that there is a small gap between the 3' end of the 5' arm and the 5' end of the 3' arm, thus the insertion of the TI{CRIMIC.TG4.0} cassette is predicted to be accompanied by a deletion of 19bp of genomic sequence flanking the insertion site.

Df(2L)BSC208/Frmd5^{CR00705-TG4.0} is rescued by Scer\GAL4^{Frmd5-CR00705-TG4.0}/Frmd5^{WT.UAS.Tag:HA}