A TI{CRIMIC.TG4.2} DNA cassette has been inserted into CG2614, in a coding intron, and is predicted to gene trap all annotated transcripts of the gene. The cassette is less than 500 bp upstream of the 5' end of the brun gene. The TI{CRIMIC.TG4.2} cassette was inserted using the CRISPR/Cas9 technique together with a donor plasmid to drive homology directed repair. The sgRNA sequence used to target the gene was: AGAAGTCTGCTCGAGCTTAGTGG. The homology arms of the donor vector overlapped by 19 bp. It is unknown whether the insertion is precise or if there is a small deletion or duplication. The insertion site coordinate is the Cas9 cut site predicted for the CRISPR guide RNA.