A TI{CRIMIC.TG4.1} DNA cassette has been inserted into Hakai, in a coding intron, and is predicted to gene trap all annotated transcripts of the gene. The predicted insertion site is very close to the splice donor of the intron or within the upstream exon of Hakai, which might affect the proper expression of GAL4. However, GAL4 expression in the optic lobe and elsewhere in the brain has been observed. The TI{CRIMIC.TG4.1} cassette was inserted using the CRISPR/Cas9 technique together with a donor plasmid to drive homology directed repair. The sgRNA sequence used to target the gene was: GAGTCTCCTCCCCTATTGAATGG. The homology arms of the donor vector overlapped by 4 bp. It is unknown whether the insertion is precise or if there is a small deletion or duplication. The insertion site coordinate is the Cas9 cut site predicted for the CRISPR guide RNA.