A TI{CRIMIC.TG4.2} DNA cassette has been inserted into qkr58E-1, in a coding intron, and is predicted to gene trap all annotated transcripts of the gene. The CRIMIC insertion is 5-10 bp from the splice acceptor junction, which might affect the effectiveness of the CRIMIC to act as a gene trap and to express GAL4. However, there is GAL4 expression in part of the brain. The TI{CRIMIC.TG4.2} cassette was inserted using the CRISPR/Cas9 technique together with a donor plasmid to drive homology directed repair. The sgRNA sequence used to target the gene was: AAAAAGTAAGGGATTAAAGGAGG. The homology arms of the donor plasmid used were designed such that there is a small gap between the 3' end of the 5' arm and the 5' end of the 3' arm, thus the insertion of the TI{CRIMIC.TG4.2} cassette is predicted to be accompanied by a deletion of 18bp of genomic sequence flanking the insertion site.