FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Sec22ERM1
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General Information
Symbol
Dmel\Sec22ERM1
Species
D. melanogaster
Name
FlyBase ID
FBal0341661
Feature type
allele
Associated gene
Dmel\Sec22
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Zhao et al., 2015)
Progenitor genotype
Cytology
Description

G to A mutation at the splice acceptor site at the end of the third intron. This results in the production of two mutant mRNAs; in one case, an alternative splice site is used, resulting in a deletion of part of the fourth exon and a frameshift that results in mistranslation of most of the synaptobrevin domain. In the other case, the transcript contains an unspliced intron that results in a premature stop codon in the synaptobrevin domain.

(Zhao et al., 2015)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
X:807,353..807,353 [+]
Nucleotide change:

G807353A

Comment:

Nucleotide substitution: AG changed to AA in the third intron splice acceptor.

(Zhao et al., 2015)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Sec22ERM1 homozygous clones within mosaic fat bodies exhibit an accumulation and enlargement of ER and late endosomes (marked by KDEL-GFP and Rab7-GFP) and exhibit a more diffuse Golgi (marked by ManII-GFP), but do not show any obvious defects in mitochondria or in starvation-induced autophagy (monitored by Atg8-GFPRFP and p62), as compared to controls.

      Sec22ERM1 homozygous clones within mosaic eyes exhibit small and irregularly shaped rhabdomeres which sometimes are fused with each other, as compared to controls; inside photoreceptor cells, the ER is highly expanded with numerous stacks and gradually loses its normal morphology with aging, and lipid droplets can be detected in the cytoplasm, as compared to controls.

      (Zhao et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Fat body clones that are both mutants for Sec22ERM1 and express either Syx5JF03330 or CG42383UAS.Tag:MYC under the control of Scer\GAL4GMR.PU show small cells, as compared to controls.

      (Zhao et al., 2015)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      Sec22ERM1 is rescued by Sec22+t3.3

      (Zhao et al., 2015)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      ERM1
      (Zhao et al., 2015)
      Sec22ERM1
      Name Synonyms
      Secondary FlyBase IDs
        References (1)