FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\shiXFRT2
Open Close
General Information
Symbol
Dmel\shiXFRT2
Species
D. melanogaster
Name
FlyBase ID
FBal0342448
Feature type
allele
Associated gene
Dmel\shi
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Fang et al., 2016)
Progenitor genotype
Cytology
Description

Amino acid replacement: K10term.

(Fang et al., 2016)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
X:15,893,032..15,893,032 [+]
Nucleotide change:

A15893032T

Reported nucleotide change:

A?T

Amino acid change:

K10term | shi-PA; K10term | shi-PB; K10term | shi-PC; K10term | shi-PE; K10term | shi-PF; K10term | shi-PG; K10term | shi-PH; K10term | shi-PI; K10term | shi-PJ; K10term | shi-PK; K10term | shi-PL; K10term | shi-PM; K10term | shi-PN; K10term | shi-PO; K10term | shi-PP

Reported amino acid change:

K10term

Comment:

The mutation is reported as R (AAG) to stop (TAG) at amino acid 10. Codon 10 is AAG but this encodes Lys (K) so it should have been reported as K10stop. Surrounding sequences show that the mutation is in amino acid 10.

(Fang et al., 2016)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      shiXFRT2 clones in the third instar larval fat body fail to induce autophagy upon starvation, as shown by their failure to increase LysoTracker levels. In agreement, under starvation conditions the clones show an accumulation and enlargement of early and late endosomes (identified as GFP-FYVE and GFP-Rab7 cytoplasmic puncta, respectively), an enlargement of lysosomes (identified as LAMP-GFP puncta) and their accumulation at the cell membrane, and an accumulation and enlargement of autophagosomes (identified as GFP-Atg8 cytoplasmic puncta and p62 cytoplasmic puncta), as compared to starved controls.

      (Fang et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      XG12A
      (Fang et al., 2016)
      shiXFRT2
      (Fang et al., 2016)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)