There may be a problem with the splicing of the "Trojan exon" intron into the mRNA because the target site of the CRIMIC insertion was mistakenly designed to be within a coding exon of the gene.

A TI{CRIMIC.TG4.0} DNA cassette has been inserted into CG17059, in a coding exon. The TI{CRIMIC.TG4.0} cassette was inserted using the CRISPR/Cas9 technique together with a donor plasmid to drive homology directed repair. The sgRNA sequence used to target the gene was: TTCGATGGGAAGCTATGTTGAGG. The homology arms of the donor plasmid used were designed such that there is a small gap between the 3' end of the 5' arm and the 5' end of the 3' arm, thus the insertion of the TI{CRIMIC.TG4.0} cassette is predicted to be accompanied by a deletion of 27bp of genomic sequence flanking the insertion site.