A TI{CRIMIC.TG4.2} DNA cassette has been inserted into shg, in a coding intron. Not predicted to express GAL4 because the reading frame of the T2A-GAL4 of the CRIMIC donor construct was the wrong phase for the annotated gene. The TI{CRIMIC.TG4.2} cassette was inserted using the CRISPR/Cas9 technique together with a donor plasmid to drive homology directed repair. The sgRNA sequence used to target the gene was: TCCCACTGTCGCACGTCCTACGG. The homology arms of the donor vector overlapped by 6 bp. It is unknown whether the insertion is precise or if there is a small deletion or duplication. The insertion site coordinate is the Cas9 cut site predicted for the CRISPR guide RNA.
