The RMCE cassette in TI{CRIMIC.GT15}ari-1T-GT15 has been replaced with an intron phase 0 protein trap cassette that is composed of a splice acceptor site, the 'GFSTF' multiple tag cassette (EGFP-Tag:CALI(TC)-Tag:StrepII-Tag:CS(TEVp)-3xTag:FLAG) and a splice donor site.
ari-1T-GFSTF.0 homozygotes display normal lifespan; body wall muscles in their third instar larvae show the typically even spaced nuclei observed in controls.