FlyBase Allele Report: Dmel\iPLA2-VIA[Δ174]

General Information

Symbol

Dmel\iPLA2-VIA^Δ174

Species

D. melanogaster

Name

FlyBase ID

FBal0343297

Feature type

allele

Associated gene

Dmel\iPLA2-VIA

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Lin et al., 2018)

Mutagen

P-element activity

(Lin et al., 2018)

Progenitor genotype

P{EPgy2}iPLA2-VIA^EY05103

(Lin et al., 2018)

Cytology

Description

Deletion removing part of the iPLA2-VIA transcription unit. Generated by imprecise excision of P{EPgy2}iPLA2-VIA^EY05103.

(Lin et al., 2018)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

delins

3L:9,862,707..9,864,041 [-]

Comment:

A 1335bp deletion ( 3L:9862707..9864041 ) resulting from the imprecise excision of P{EPgy2}iPLA2-VIA^EY05103 removes the 5' end of iPLA2-VIA. The sequence "CATGATGAAATAACACGTACACGTATACACGTATAACA" is inserted at the site of the deletion.

Inserted_sequence:

CATGATGAAATAACACGTACACGTATACACGTATAACA

(Lin and Bellen, 2019.2.8)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Parkinson's disease 14

CEA

(Lin et al., 2018)

model of neurodegeneration with brain iron accumulation 2a

CEA

(Lin et al., 2023)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of Parkinson's disease 14

is ameliorated by Vps26^UAS.Tag:HA

(Lin et al., 2018)

is ameliorated by Vps35^UAS.Tag:HA

(Lin et al., 2018)

is ameliorated by lace^KK102282

(Lin et al., 2018)

is ameliorated by Hsap\PLA2G6^UAS.cLa

(Lin et al., 2018)

is ameliorated by Hsap\PLA2G6^S519A.UAS

(Lin et al., 2018)

model of neurodegeneration with brain iron accumulation 2a

is ameliorated by iPLA2-VIA^{+CH322-150H21}

(Lin et al., 2023)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

iPLA2-VIA^Δ174 mutants show lower performance in odor avoidance behavior (to 3-octanol or 4-methylcyclohexanol).

These mutants show progressive functional and ultrastructure eye defects. There are decreases in electroretinogram amplitudes and in on-transients electroretinogram amplitude; the decreased on-transients electroretinogram amplitude, but not the electroretinogram amplitude is more severe under 12h:12h light;dark cycles than under constant darkness. There is a progressive loss and degeneration of photoreceptors: aberrant photoreceptor cell morphology, swollen postsynaptic dendrites, expanded presynaptic terminals, a decrease in the density of synaptic vesicles, the accumulation of TSV-like structures and omega-like structures, a decrease in capitate projections and an accumulation of lysosomes; there is also an accumulation of aberrant mitochondria by day 30.

The third instar larval fat body in iPLA2-VIA^Δ174 mutants show larger and more numerous lysosomes (identified as Lysotracker-positive puncta), as compared to controls.

(Lin et al., 2018)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

iPLA2-VIA^Δ174 has bang sensitive phenotype, suppressible by Scer\GAL4^Act5C.PI/Vps26^UAS.Tag:HA

(Lin et al., 2018)

iPLA2-VIA^Δ174 has bang sensitive phenotype, suppressible | partially by Vps35^UAS.Tag:HA/Scer\GAL4^Act5C.PI