FlyBase Allele Report: Dmel\ATP8A[sgRNA.U6]

General Information

Symbol

Dmel\ATP8A^sgRNA.U6

Species

D. melanogaster

Name

FlyBase ID

FBal0343395

Feature type

allele

Associated gene

Dmel\ATP8A

Associated Insertion(s)

Carried in Construct

PBac{U6-ATP8A.sgRNA}

Also Known As

gRNA-ATP8A

Key Links

Transgenic product class

sgRNA

(Sapar et al., 2018)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

sgRNA

(Sapar et al., 2018)

Mutagen

in vitro construct

(Sapar et al., 2018)

Progenitor genotype

Carried in construct

PBac{U6-ATP8A.sgRNA}

Cytology

Description

CRISPR construct designed to knockout the ATP8A gene when combined with a source of Cas9 endonuclease. Constitutively expresses two sgRNAs, both of which target coding exon sequence that is shared by all ATP8A splice isoforms.

(Sapar et al., 2018)

Allele components

Component

Use(s)

Encoded product / tool

ATP8A

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

larval multidendritic class IV neuron | larval stage, with Spyo\Cas9^ppk.PS

(Sapar et al., 2018)

Detailed Description

Statement

Reference

The knockout of ATP8A in class IV dendritic arborizing neurons by the combination of ATP8A^sgRNA.U6 and Spyo\Cas9^ppk.PS leads to some dendrite debris being detected on the underlying epidermal cells, but does not affect dendrite density or dendrite dendrite field size.

(Sapar et al., 2018)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

ATP8A^sgRNA.U6, Spyo\Cas9^ppk.PS has larval multidendritic class IV neuron | larval stage phenotype, non-enhanceable by Cdc50^sgRNA.U6, Spyo\Cas9^ppk.PS

(Sapar et al., 2018)

NOT suppressed by

Statement

Reference

ATP8A^sgRNA.U6, Spyo\Cas9^ppk.PS has larval multidendritic class IV neuron | larval stage phenotype, non-suppressible by Cdc50^sgRNA.U6, Spyo\Cas9^ppk.PS

(Sapar et al., 2018)

NOT Enhancer of

Statement

Reference

ATP8A^sgRNA.U6, Spyo\Cas9^ppk.PS is a non-enhancer of larval multidendritic class IV neuron | larval stage phenotype of Cdc50^sgRNA.U6, Spyo\Cas9^ppk.PS

(Sapar et al., 2018)

NOT Suppressor of

Statement

Reference

ATP8A^sgRNA.U6, Spyo\Cas9^ppk.PS is a non-suppressor of larval multidendritic class IV neuron | larval stage phenotype of Cdc50^sgRNA.U6, Spyo\Cas9^ppk.PS

(Sapar et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

The tissue-specific double knockout of CDC50 and ATP8A (CDC50^sgRNA.U6+ATP8A^sgRNA.U6+Spyo\Cas9^ppk.PS) in class IV dendritic arborizing neurons does not affect dendrite density or dendrite dendrite field size, as compared to controls.

(Sapar et al., 2018)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

ATP8A^sgRNA.U6

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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