FB2026_02 , released June 18, 2026
FB2026_02 , released June 18, 2026
Allele: Dmel\dacΔPRE2.ΔPRE1
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General Information
Symbol
Dmel\dacΔPRE2.ΔPRE1
Species
D. melanogaster
Name
FlyBase ID
FBal0343451
Feature type
allele
Associated gene
Dmel\dac
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Ogiyama et al., 2018)
gene targeting by homologous recombination
(Ogiyama et al., 2018)
Progenitor genotype
Cytology
Description

645bp deletion that removes a Polycomb response element (PRE) located close to the 5' end of the dac gene, plus an approximately 1kb deletion that removes a Polycomb response element (PRE) located 70kb 3' to dac

(Ogiyama et al., 2018)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
delins
2L:16,485,898..16,486,571 [-]
Comment:

A 674bp region is replaced with 118 bases (ATACGTACACACGAAGCGCTACCGTTGCCTTATGCATGGAGATCTTTACTAGTGCTCTTCTATAACTTCGTATAGCATACATTATACGAAGTTATGATCGCAGGTGTGCATATGTCCG). One of two mutations in strain.

Inserted_sequence:

ATACGTACACACGAAGCGCTACCGTTGCCTTATGCATGGAGATCTTTACTAGTGCTCTTCTATAACTTCGTATAGCATACATTATACGAAGTTATGATCGCAGGTGTGCATATGTCCG

(Ogiyama et al., 2018)
delins
2L:16,422,436..16,423,525 [-]
Comment:

A 1090bp region is replaced with 97bp (GCGGACATATGCACACCTGCGATCATAACTTCGTATAATGTATGCTATACGAAGTTATAGAAGAGCACTAGTAAAGATCTCCATGCATAAGGCGCGC). One of two deletions in mutant strain. This deletion maps downstream of dac within the large intron of CG5888. The other maps just upstream of dac.

Inserted_sequence:

GCGGACATATGCACACCTGCGATCATAACTTCGTATAATGTATGCTATACGAAGTTATAGAAGAGCACTAGTAAAGATCTCCATGCATAAGGCGCGC

(Ogiyama et al., 2018)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      dacΔPRE2.ΔPRE1 homozygous adult males frequently show an ectopic sex comb on the T2 prothoracic leg segment; the phenotype progresses to almost full penetrance from 21[o]C to 28[o]C.

      (Ogiyama et al., 2018)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Statement
      Reference

      dacΔPRE2.ΔPRE1 has visible | male phenotype, enhanceable by ph-p410

      (Ogiyama et al., 2018)
      Phenotype Manifest In
      Enhanced by
      Statement
      Reference

      dacΔPRE2.ΔPRE1 has sex comb | ectopic phenotype, enhanceable by ph-p410

      (Ogiyama et al., 2018)

      dacΔPRE2.ΔPRE1 has prothoracic tarsal segment 2 | male phenotype, enhanceable by ph-p410

      (Ogiyama et al., 2018)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The ectopic sex comb phenotype observed in dacΔPRE2.ΔPRE1 homozygotes is enhanced to full penetrance by ph-p410 at 21[o[C] and 25[o]C; a nearly fully penetrant phenotype is also observed when dacΔPRE2.ΔPRE1 is in heterozygosity.

      (Ogiyama et al., 2018)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      dacΔPRE2.ΔPRE1
      ΔPRE2,ΔPRE1
      (Ogiyama et al., 2018)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)