FlyBase Allele Report: Dmel\Rif1[KO]

General Information

Symbol

Dmel\Rif1^KO

Species

D. melanogaster

Name

FlyBase ID

FBal0344229

Feature type

allele

Associated gene

Dmel\Rif1

Associated Insertion(s)

TI{TI}Rif1^KO

Carried in Construct

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

CRISPR/Cas9

(Seller and O'Farrell, 2018)

gene targeting by homologous recombination

(Seller and O'Farrell, 2018)

Progenitor genotype

Associated Insertion(s)

TI{TI}Rif1^KO

Cytology

Description

Precise deletion of the Rif1 open reading frame. A Disc\RFP^{DsRed.3xP3.cUa} marker has been inserted in place of the deleted sequence.

(Seller and O'Farrell, 2018)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle | embryonic stage | maternal effect

(Seller and O'Farrell, 2018)

abnormal mitotic cell cycle | embryonic stage | maternal effect (with Rif1^EGFP)

(Cho et al., 2022)

fertile

(Cho et al., 2022, Seller and O'Farrell, 2018)

partially lethal

(Seller and O'Farrell, 2018)

some die during embryonic stage | maternal effect

(Seller and O'Farrell, 2018)

viable

(Cho et al., 2022)

Phenotype Manifest In

nucleus | embryonic stage | maternal effect

(Seller and O'Farrell, 2018)

Detailed Description

Statement

Reference

Rif1^KO homozygous mutants develop to adulthood with a reduced survival rate and a reduced male-to-female ratio. Embryos laid by Rif1^KO/Rif1^KO mothers have a reduced hatch rate as well as cell cycle defects; including a shorter mitotic S phase 13, a significantly shorter S phase 14, resembling earlier S phases, and occasional early entry into (an additional) mitosis 14, which completes, but with substantial nuclear fall in.

(Seller and O'Farrell, 2018)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Rif1^KO has some die during embryonic stage | maternal effect phenotype, enhanceable | maternal effect by Cdc7^GL00585/Scer\GAL4^{VP16.mat.αTub67C}

(Seller and O'Farrell, 2018)

Suppressor of

Statement

Reference

Rif1^KO/Rif1^KO is a suppressor | maternal effect | partially of majority die during embryonic stage | maternal effect phenotype of Cdc7^GL00585, Scer\GAL4^{VP16.mat.αTub67C}

(Seller and O'Farrell, 2018)

Rif1^KO/Rif1^KO is a suppressor | maternal effect | partially of abnormal mitotic cell cycle | maternal effect | embryonic stage phenotype of Cdc7^GL00585, Scer\GAL4^{VP16.mat.αTub67C}

(Seller and O'Farrell, 2018)

Phenotype Manifest In

Suppressor of

Statement

Reference

Rif1^KO/Rif1^KO is a suppressor | maternal effect | partially of nucleus | maternal effect | embryonic stage phenotype of Cdc7^GL00585, Scer\GAL4^{VP16.mat.αTub67C}

(Seller and O'Farrell, 2018)

Additional Comments

Genetic Interactions

Statement

Reference

Rif1^KO homozygosity, but not heterozygosity in mothers expressing Cdc7^GL00585 under the control of Scer\GAL4^{VP16.mat.αTub67C} restores cell cycle progression, with most embryos reaching the blastoderm stage; nuclear abnormalities still occur, but some embryos (a minority) survive and hatch.

(Seller and O'Farrell, 2018)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Rif1^KO

(Cho et al., 2022)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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