FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\sdtGFP3
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General Information
Symbol
Dmel\sdtGFP3
Species
D. melanogaster
Name
FlyBase ID
FBal0345004
Feature type
allele
Associated gene
Dmel\sdt
Associated Insertion(s)
TI{TI}sdtGFP3
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
gene targeting by homologous recombination
(Perez-Mockus et al., 2017)
CRISPR/Cas9
(Perez-Mockus et al., 2017)
Progenitor genotype
Associated Insertion(s)
TI{TI}sdtGFP3
Cytology
Description

GFP is inserted within exon 3 of sdt (amino acid 188 of sdt-PB), along with two FRT sites inserted into the introns flanking exon 3. GFP thus tags all six neur binding motif-containing (NBM) sdt isoforms as well as three isoforms that contain only part of exon 3, excluding the NBM.

(Perez-Mockus et al., 2017)
Allele components
Component
Use(s)
Tagged with
GFP
Also carries
FRT
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
insertion
X:8,225,220..8,225,221 [+]
Comment:

Avic\GFP is inserted within exon 3 of sdt (at amino acid 188 of sdt-PB - exact site unspecified), along with two FRT sites inserted into the introns flanking exon 3.

(Perez-Mockus et al., 2017)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Stage 9/10 sdtGFP3 embryos do not show any obvious defects in epithelial integrity or apical-basal cell polarity (assessed by the localization of aPKC and Nrt proteins). In mutant eye discs, ommatidia form the expected six-cell rosettes.

      (Perez-Mockus et al., 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Stage 9/10 Df(3L)Brd-C1/Df(3R)E(spl)δ-6 transheterozygous embryos display loss of apical-basal cell polarity and of epithelial integrity; these are not rescued by sdtGFP3.

      Stage 9/10 Df(3L)Brd-C1/Df(3R)E(spl)δ-6 transheterozygous embryos display loss of apical-basal cell polarity and of epithelial integrity; none of these defects is rescued by sdtGFP3.

      (Perez-Mockus et al., 2017)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      sdtGFP3
      (Shard et al., 2020, Perez-Mockus et al., 2017)
      Name Synonyms
      Secondary FlyBase IDs
        References (2)