A TI{CRIMIC.TG4.0} DNA cassette has been inserted into Jhe using the CRISPR/Cas9 technique together with a donor plasmid to drive homology directed repair. The cassette is not predicted to trap Jhe or express GAL4 because the CRISPR sgRNA was mistakenly targeted to a coding exon of the gene. However, GAL4 expression is seen in the larval and adult nervous system. The sgRNA sequence used to target the gene was: GCCTGTAGACATTCAAGTAGAGG. The homology arms of the donor plasmid used were designed such that there is a small gap between the 3' end of the 5' arm and the 5' end of the 3' arm, thus the insertion of the TI{CRIMIC.TG4.0} cassette is predicted to be accompanied by a deletion of 13bp of genomic sequence flanking the insertion site.