A TI{CRIMIC.TG4.1} DNA cassette has been inserted into Unc-89, using the CRISPR/Cas9 technique together with a donor plasmid to drive homology directed repair. The cassette is not predicted to trap Unc-89 or express GAL4 because the CRISPR sgRNA was mistakenly targeted to a coding exon of the gene; no GAL4 expression was detected in the larval or adult nervous system. The sgRNA sequence used to target the gene was: GCTTTACGGATGGCCAGAGTCGG. The homology arms of the donor plasmid used were designed such that there is a small gap between the 3' end of the 5' arm and the 5' end of the 3' arm, thus the insertion of the TI{CRIMIC.TG4.1} cassette is predicted to be accompanied by a deletion of 665bp of genomic sequence flanking the insertion site.