FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\PzlU6.sgRNA
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General Information
Symbol
Dmel\PzlU6.sgRNA
Species
D. melanogaster
Name
FlyBase ID
FBal0346231
Feature type
allele
Associated gene
Dmel\Pzl
Associated Insertion(s)
Carried in Construct
P{U6-pzl.sgRNA}
Key Links
Transgenic product class
sgRNA
(Hu et al., 2019)
Mutagen
Nature of the Allele
Transgenic product class
sgRNA
(Hu et al., 2019)
Mutagen
in vitro construct
(Hu et al., 2019)
Progenitor genotype
Carried in construct
P{U6-pzl.sgRNA}
Cytology
Description

U6 promoters drive expression of 4 different sgRNAs, that each target Pzl.

(Hu et al., 2019)
Allele components
Component
Use(s)
Encoded product / tool
Pzl
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Knock-out of Pzl in Cho neurons PzlU6.sgRNA and expressing Spyo\Cas9UAS.Tag:NLS(Unk) under the control of Scer\GAL4iav.PP induces abnormal locomotor behavior, with severely increased periods of head lifting and spontaneous backward movements compared to controls, as well as severely higher frequency of bending their bodies laterally to greater than 90[o], which is rarely observed in wild-type controls.

      (Hu et al., 2019)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      PzlU6.sgRNA
      Name Synonyms
      Secondary FlyBase IDs
        References (1)