FB2026_02 , released June 18, 2026
Allele: Dmel\DlishB1601
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General Information
Symbol
Dmel\DlishB1601
Species
D. melanogaster
Name
FlyBase ID
FBal0346822
Feature type
allele
Associated gene
Dmel\Dlish
Associated Insertion(s)
TI{mCherry}DlishB1601
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Wang et al., 2019)
gene targeting by homologous recombination
(Wang et al., 2019)
Progenitor genotype
Associated Insertion(s)
TI{mCherry}DlishB1601
Cytology
Description

Deletion (coordinates 2R:17723433..17724834 ) that removes the sequence coding for Dlish amino acid residues 1-350. The following sequence (from the pTV-Cherry-based donor template) has been inserted in place of the deleted sequence: a single attP site and a loxP cassette which itself contains an mFRT71 cassette followed by Scer\GAL4 coding sequence and a polyadenylation signal (this GAL4 sequence is non-functional). The mFRT71 cassette contains 1: the mCherry coding region tagged with a membrane-targeting signal (Tag:Myr(Unk)) and followed by a polyadenylation signal, 2: a w+* marker. The mCherry coding region is expressed under the control of the endogenous Dlish regulatory sequences.

(Wang et al., 2019)
Allele components
Component
Use(s)
Inserted element
TI{mCherry}
Encoded product / tool
mCherry
Also carries
attP
loxP
mFRT71
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
delins
2R:17,723,433..17,724,834 [+]
Comment:

Sequences encoding amino acides 1-350 of Dlish have been deleted and replaced with a single attP site and a loxP cassette which itself contains an mFRT71 cassette followed by Scer\GAL4 coding sequence and a polyadenylation signal (this GAL4 sequence is non-functional). The mFRT71 cassette contains 1: the mCherry coding region tagged with a membrane-targeting signal (Tag:Myr(Unk)) and followed by a polyadenylation signal, 2: a w+* marker. The mCherry coding region is expressed under the control of the endogenous Dlish regulatory sequences.

(Wang et al., 2019)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      DlishB1601
      dlishB1601
      (Fulford et al., 2023, Wang et al., 2019)
      Name Synonyms
      Secondary FlyBase IDs
        References (2)