FlyBase Allele Report: Dmel\His3.3B[K9R]

FB2026_01 , released March 12, 2026

FB2026_01 , released March 12, 2026

Allele: Dmel\His3.3B^K9R

General Information

Symbol

Dmel\His3.3B^K9R

Species

D. melanogaster

Name

FlyBase ID

FBal0347474

Feature type

allele

Associated gene

Associated Insertion(s)

Carried in Construct

Also Known As

H3.3B^K9R

Key Links

Genomic Maps

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

(Penke et al., 2018)

gene targeting by homologous recombination

(Penke et al., 2018)

Progenitor genotype

Cytology

Description

The amino acid substitution K9R has been introduced into the endogenous His3.3B locus.

(Penke et al., 2018)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

X:9,154,633..9,154,633 [-]

Nucleotide change:

A9154633G

Amino acid change:

K10R | His3.3B-PA; K10R | His3.3B-PB; K10R | His3.3B-PC; K10R | His3.3B-PE

Reported amino acid change:

K9R

Comment:

The amino acid substitution "K9R" has been introduced into the endogenous His3.3B locus. The K is actually the 10th amino acid since the initiator Met does not appear to be counted in the numbering. Site of nucleotide substitution in mutant inferred by FlyBase curator based on reported amino acid change.

(Penke et al., 2018)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

(Penke et al., 2018)

sterile, with His3.3B^K9R.tg

(Penke et al., 2018)

(Penke et al., 2018)

Phenotype Manifest In

(Penke et al., 2018)

chromosome, telomeric region

(Penke et al., 2018)

polytene chromosome

(Penke et al., 2018)

polytene chromosome chromocenter

(Penke et al., 2018)

(Penke et al., 2018)

Detailed Description

Statement

Reference

His3.3B^K9R homozygotes have an increased proportion of disorganized chromocenters in polytene chromosomes compared to controls.

Chromosomes in wing discs of His3.3B^K9R homozygotes have a disrupted chromatin architecture.

(Penke et al., 2018)

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

His3.3A^2X1/Df(2L)BSC110, His3.3B^K9R has sterile phenotype, suppressible by His3.3B^+t5

(Penke et al., 2018)

Other

Statement

Reference

His3.3A[+]/His3.3A^2X1, His3.3B^K9R has viable phenotype

(Penke et al., 2018)

His3.3A[+]/His3.3A^2X1, His3.3B^K9R has fertile phenotype

(Penke et al., 2018)

Df(2L)His^C, His3.3A^2X1, His3.3B^K9R, His3^HisTA.H3K9R has lethal - all die before end of larval stage phenotype

(Penke et al., 2018)

His3.3A^2X1/Df(2L)BSC110, His3.3B^K9R has viable phenotype

(Penke et al., 2018)

His3.3A^2X1/Df(2L)BSC110, His3.3B^K9R has sterile phenotype

(Penke et al., 2018)

Df(2L)His^C, His3.3A^2X1, His3.3B^K9R, His3^HisTA.wt/His3[+] has partially lethal - majority die phenotype

(Penke et al., 2018)

Phenotype Manifest In

Other

Statement

Reference

His3.3A^2X1/Df(2L)BSC110, His3.3B^K9R has wing disc phenotype

(Penke et al., 2018)

His3.3A^2X1/Df(2L)BSC110, His3.3B^K9R has chromatin phenotype

(Penke et al., 2018)

His3.3A^2X1/Df(2L)BSC110, His3.3B^K9R has chromosome, telomeric region phenotype

(Penke et al., 2018)

His3.3A^2X1/Df(2L)BSC110, His3.3B^K9R has polytene chromosome phenotype

(Penke et al., 2018)

His3.3A^2X1/Df(2L)BSC110, His3.3B^K9R has polytene chromosome chromocenter phenotype

(Penke et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

The sterility of His3.3B^K9R/His3.3B^K9R; His3.3A^2X1/Df(2L)BSC110 males and females is rescued by His3.3B^+t5.

(Penke et al., 2018)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

H3.3B^K9R

(Armstrong et al., 2018, Penke et al., 2018)

His3.3B^K9R

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections