FB2026_02 , released June 18, 2026
Allele: Dmel\kisK2060R.UAS.L.Tag:polyHis,Tag:FLAG
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General Information
Symbol
Dmel\kisK2060R.UAS.L.Tag:polyHis,Tag:FLAG
Species
D. melanogaster
Name
FlyBase ID
FBal0351581
Feature type
allele
Associated gene
Dmel\kis
Associated Insertion(s)
Carried in Construct
PBac{UAS-kis.L.K2060R}
Key Links
Transgenic product class
missense_variant
(Gervais et al., 2019)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Gervais et al., 2019)
Mutagen
in vitro construct
(Gervais et al., 2019)
Progenitor genotype
Carried in construct
PBac{UAS-kis.L.K2060R}
Cytology
Description

UAS regulatory sequences drive expression of the kis-RA (long) isoform, mutated to carry a K2060R amino acid substitution in the highly conserved ATP binding motif; this is expected to result in an an ATPase-dead version of the protein. The coding sequence is tagged at the N-terminal end with Tag:polyHis and at the C-terminal end with Tag:FLAG.

(Gervais et al., 2019)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
kis
Tagged with
Tag:FLAG
Tag:polyHis
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      kisK2060R.UAS.L.Tag:polyHis,Tag:FLAG/Scer\GAL4Tub.PU partially rescues kis10D26

      (Gervais et al., 2019)
      Comments

      Expressing kisK2060R.UAS.L.Tag:polyHis,Tag:FLAG under the control of Scer\GAL4Tub.PU partially rescues the increased overall number of cells and of intestinal stem cells in somatic clones homozygous for kis10D26.

      (Gervais et al., 2019)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      kisK2060R.UAS.L.Tag:polyHis,Tag:FLAG
      Name Synonyms
      Secondary FlyBase IDs
        References (1)