A Crk rescue construct, consisting of FRT-flanked Crk cDNA (corresponding to Crk-RA) tagged at the N-terminus with mNeonGreen and 3xTag:FLAG, inserted in the TI{TI}CrkΔattP locus. (The FRT sites are inserted 104 bp upstream of the transcription start site and 99 bp downstream of the end of the 3' UTR, and allow deletion via the FLP recombinase to facilitate production of females whose germlines are homozygous mutant.)