FlyBase Allele Report: Dmel\Zasp66[CRISPR]

General Information

Symbol

Dmel\Zasp66^CRISPR

Species

D. melanogaster

Name

FlyBase ID

FBal0353077

Feature type

allele

Associated gene

Dmel\Zasp66

Associated Insertion(s)

TI{GAL4::VP16}Zasp66^CRISPR

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

CRISPR/Cas9

(González-Morales et al., 2019)

gene targeting by homologous recombination

(González-Morales et al., 2019)

Progenitor genotype

Associated Insertion(s)

TI{GAL4::VP16}Zasp66^CRISPR

Cytology

Description

Deletion within Zasp66; exons 2, 3, 4 and part of exon 5 are replaced with a cassette encoding an attP site, GAL4::VP16 driver coding sequence, and a loxP cassette that contains a w^+* marker.

(González-Morales et al., 2019)

Allele components

Component

Use(s)

Inserted element

TI{GAL4::VP16}

promoter trap

Encoded product / tool

GAL4::VP16

binary expression system - driver

Also carries

attP

integrase target site

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

delins

3L:8,626,424..8,630,587 [+]

Comment:

Approximate deletion breakpoints determined from reported guide RNA sequences; exons 2, 3, 4 and part of exon 5 of Zasp66 are replaced with a cassette encoding an attP site, a GAL4::VP16 driver coding sequence, and a loxP cassette that contains a w^+* marker.

(González-Morales et al., 2019)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

majority die during P-stage

(González-Morales et al., 2019)

partially lethal - majority die

(González-Morales et al., 2019)

Phenotype Manifest In

indirect flight muscle cell

(González-Morales et al., 2019)

Z disc | adult stage

(González-Morales et al., 2019)

Detailed Description

Statement

Reference

Zasp66^CRISPR/+ and Zasp66^CRISPR/Zasp66^CRISPR adults do not show flight or climbing defects. Zasp66^CRISPR/Zasp66^CRISPR adults show few obvious indirect flight muscle defects.

(González-Morales et al., 2019)

The indirect flight muscles of Zasp66^CRISPR homozygotes show Z-disc aggregation, although Z-disc size is unaffected.

(González-Morales et al., 2019)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Zasp66^CRISPR has indirect flight muscle cell phenotype, enhanceable by Zasp67^CRISPR/Zasp67^CRISPR

(González-Morales et al., 2019)

Zasp66^CRISPR has Z disc | adult stage phenotype, enhanceable by Zasp67^CRISPR/Zasp67^CRISPR

(González-Morales et al., 2019)

Enhancer of

Statement

Reference

Zasp66^CRISPR/Zasp66^CRISPR is an enhancer of indirect flight muscle cell phenotype of Zasp67^CRISPR

(González-Morales et al., 2019)

Zasp66^CRISPR/Zasp66^CRISPR is an enhancer of Z disc | adult stage phenotype of Zasp67^CRISPR

(González-Morales et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

Double Zasp66^CRISPR, Zasp67^CRISPR homozygotes show more a severe Z-disc aggregation in indirect flight muscles than either single homozygous mutant. The z-discs of doublemutants are also enlarged, as compared to either single mutant or wild-type controls.

(González-Morales et al., 2019)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

Zasp66^CRISPR is partially rescued by Zasp66^{+tCH322-127P23}

(González-Morales et al., 2019)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Zasp66^CRISPR

Zasp66^KO

(González-Morales et al., 2019)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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