FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\RhoGEF2DHPH.UASp.CRY2(PHR),mCherry
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General Information
Symbol
Dmel\RhoGEF2DHPH.UASp.CRY2(PHR),mCherry
Species
D. melanogaster
Name
FlyBase ID
FBal0355318
Feature type
allele
Associated gene
Dmel\RhoGEF2
Associated Insertion(s)
Carried in Construct
P{UASp-RhoGEF2.DHPH.CRY2::mCherry}
Also Known As
UASp>RhoGEF2-CRY2::mCherry
Key Links
Transgenic product class
inframe_deletion
(Izquierdo et al., 2018)
Mutagen
Nature of the Allele
Transgenic product class
inframe_deletion
(Izquierdo et al., 2018)
Mutagen
in vitro construct
(Izquierdo et al., 2018)
Progenitor genotype
Carried in construct
P{UASp-RhoGEF2.DHPH.CRY2::mCherry}
Cytology
Description

UASp regulatory sequences drive expression of the RhoGEF2 DHPH catalytic domain (amino acid residues 1177-1554) that is tagged at the C-terminal end with CRY2(PHR) followed by mCherry.

(Izquierdo et al., 2018)
Allele components
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
RhoGEF2
Tagged with
CRY2(PHR)
mCherry
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      When embryos expressing RhoGEF2DHPH.UASp.CRY2(PHR),mCherry under the control of Scer\GAL4VP16.osk are exposed to uniform blue light, they exhibit dramatic dampening both of cortical contractions and of mid-embryo cytoplasmic flows, failure of nuclei to spread along the AP axis during interphase, impaired nuclear spreading (upon illumination during cycles 4 to 8), and anaphase delays when compared to controls.

      Upon light-mediated recruitment of RhoGEF2 to the anterior

      pole of the embryo expressing RhoGEF2DHPH.UASp.CRY2(PHR),mCherry under the control of Scer\GAL4VP16.osk, cortical flows are redirected toward the anterior pole; mid-embryo flows display unidirectional flows toward the posterior side of the embryo; and nuclei are consequently shuttled to the posterior side of the embryo when compared to controls.

      (Deneke et al., 2019)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Photogenetic activation/recruitment of RhoGEF2DHPH.UASp.CRY2(PHR),mCherry to membrane-tethered Atha\CIB1CIBN(ΔNLS).UASp.EGFP.Tag:PM(hKRAS) during embryo cellularization increases actomyosin contraction in all phases, although it is stronger in the priming and ring phases than in the hexagonal phase. Upon laser ablation, the initial recoil velocity and maximum tissue displacement are significantly increased during the ring phase, but not during the hexagonal phase.

      In Df(3R)Exel6218 homozygotes, in which actomyosin fibers do not organize in a hexagonal array and instead constrict prematurely, the same photogenetic activation during early cellularization also leads to increased actomyosin contraction compared to non-activated mutants.

      (Krueger et al., 2019)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      RhoGEF2DHPH.UASp.CRY2(PHR),mCherry
      Name Synonyms
      Secondary FlyBase IDs
        References (9)