[100B5-100B5];[100C1-100C1];
A set of isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Initial set of 519 isogenic deletions provides 56% genome coverage.
The current Exelixis collection at the Bloomington Stock Center differs from the original described in FBrf0175003 : a significant number were shown not to carry a deletion and have been removed from the collection; a number of stocks have been lost; a number of additional deletions are included that were generated after publication.
100B2;100B4
100B5;100C1
Breakpoint based on release 3 sequence coordinate from Parks et al., 2004 (FBrf0174230), converted to release 5 coordinate.
Breakpoint from FlyBase's release 5 sequence location of progenitor insertion.
Df(3R)Exel6218 homozygous cellularizing embryos do not assemble the typical hexagonal actomyosin network, and prematurely round and constrict. On STED super-resolution microscopy, actin filaments fail to acquire the hexagonal conformation observed in controls and instead localize in a diffuse meshwork occupying the space separating individual nuclei; actin fibers appear less thick than in controls.
Df(3R)Exel6218 homozygous embryos also fail to undergo the characteristic cell shape changes that drive ventral furrow invagination during gastrulation.
The basal fluid secretion rate of Malphigian tubules from pHCl-2Δ/Df(3R)Exel6218 adult females is higher than in wild-type.
Flies heterozygous for the deletion do not show a Minute bristle phenotype.
Limits computationally determined from location of progenitor P insertion on genome sequence between P{PZ}Aph-407028&P{PZ}dcorK215 and P{PZ}l(3)rM731rM731