The RMCE cassette of the Mi{MIC}CamtaMI04570 insertion has been replaced with a Trojan T2A-GAL4 gene trap cassette, resulting in a mutant allele of Camta, and expression of GAL4 under the control of endogenous Camta regulatory sequences.
Courtship song generated by Camtacro/CamtaMI04570-TG4.1 but not CamtaMI04570-TG4.1/+ exhibit significant prolongation of the interpulse interval with significantly higher percentage of polycyclic pulses when compared to controls.
The carrier frequency of Camtacro/CamtaMI04570-TG4.1 pulse courtship song is shifted to higher frequencies even in monocyclic pulses, though the magnitude of shift is smaller than in polycyclic pulses, and that multiple peaks at the higher frequency range are derived from polycyclic pulses when compared to controls.