FlyBase Allele Report: Dmel\polo[T182A.UASp.RR.GFP]

General Information

Symbol

Dmel\polo^{T182A.UASp.RR.GFP}

Species

D. melanogaster

Name

FlyBase ID

FBal0356541

Feature type

allele

Associated gene

Dmel\polo

Associated Insertion(s)

Carried in Construct

P{UASp-polo.T182A.RR.GFP}

Key Links

Transgenic product class

missense_variant

(Kachaner et al., 2017)

polypeptide_post_translational_processing_variant

(Kachaner et al., 2017)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Kachaner et al., 2017)

polypeptide_post_translational_processing_variant

(Kachaner et al., 2017)

Mutagen

in vitro construct

(Kachaner et al., 2017)

Progenitor genotype

Carried in construct

P{UASp-polo.T182A.RR.GFP}

Cytology

Description

UASp regulatory sequences drive expression of polo that carries a T182A amino acid replacement (mutation of the activation loop phosphorylation site in the kinase domain). The coding sequence is tagged at the C-terminal end with GFP. Synonymous codon replacements have been introduced into the transgene, so that it is insensitive to RNAi mediated by P{GD7563}.

(Kachaner et al., 2017)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

polo

Tagged with

GFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased occurrence of cell division | third instar larval stage, with Scer\GAL4^insc-Mz1407

(Gallaud et al., 2022)

Phenotype Manifest In

larval neuroblast | third instar larval stage, with Scer\GAL4^insc-Mz1407

(Gallaud et al., 2022)

Detailed Description

Statement

Reference

The expression of polo^{T182A.UASp.RNAi-res.GFP} under the control of Scer\GAL4^insc-Mz1407 induces increased mitosis time in third instar larval neuroblasts.

(Gallaud et al., 2022)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Scer\GAL4^insc-Mz1407/polo^{T182A.UASp.RR.GFP} is an enhancer of decreased occurrence of cell division | third instar larval stage phenotype of aurA⁸⁸³⁹

(Gallaud et al., 2022)

NOT Enhancer of