FlyBase Allele Report: Dmel\Jarid2[j2B8]

General Information

Symbol

Dmel\Jarid2^j2B8

Species

D. melanogaster

Name

FlyBase ID

FBal0357396

Feature type

allele

Associated gene

Dmel\Jarid2

Associated Insertion(s)

P{lacW}Jarid2^j2B8

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Silva et al., 2007)

Mutagen

P-element activity

(Spradling et al., 1999, FlyBase, 1992-)

Progenitor genotype

Associated Insertion(s)

P{lacW}Jarid2^j2B8

Cytology

Description

The P{lacW}Jarid2^j2B8 insertion is located in the intergenic region between the promoters of the divergently transcribed Uch-L5 and Jarid2 genes. Expression of Uch-L5 mRNA is unaffected in P{lacW}Jarid2^j2B8 homozygotes (when analysed by RT-PCR), while mRNA expression of both Jarid2 and the pall gene (located downstream of Jarid2) is abolished, indicating that the insertion results in a double loss of function, of both Jarid2 and pall.

(Silva et al., 2007)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:9,477,034..9,477,034 [+]

(Silva et al., 2007, Beaton, 2000.1.31, Spradling et al., 1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Beaton, 2000.1.31, Spradling et al., 1999)

partially lethal - majority die | recessive

(Berkeley Drosophila Genome Project, 1993.6.1)

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Jarid2^j2B8, pall^j2B8 has visible | recessive phenotype

(Berkeley Drosophila Genome Project, 1993.6.1)

Phenotype Manifest In

Other

Statement

Reference

Jarid2^j2B8, pall^j2B8 has leg | pharate adult stage phenotype

(Ren et al., 2005)

Jarid2^j2B8, pall^j2B8 has wing | P-stage phenotype

(Ren et al., 2005)

Jarid2^j2B8, pall^j2B8 has wing hair | P-stage phenotype

(Ren et al., 2005)

Jarid2^j2B8, pall^j2B8 has fourth segment of antenna | pharate adult stage phenotype

(Ren et al., 2005)

Jarid2^j2B8, pall^j2B8 has arista | pharate adult stage phenotype

(Ren et al., 2005)

Jarid2^j2B8, pall^j2B8 has tarsal segment | pharate adult stage phenotype

(Ren et al., 2005)

Jarid2^j2B8, pall^j2B8 has leg joint | pharate adult stage phenotype

(Ren et al., 2005)

Jarid2^j2B8, pall^j2B8 has eye phenotype

(Berkeley Drosophila Genome Project, 1993.6.1)

Additional Comments

Genetic Interactions

Statement

Reference

Homozygous pharate adults have defects such as loss of tarsal leg joints, shorter and fatter leg segments than normal, the loss of a discrete antennal segment 4 and a fatter arista than normal. Homozygous pupal wings are wider and shorter than normal and have regions with a loss of hair. (FlyBase curator comment: it is not known whether these phenotypes are caused by an effect on Jarid2 or pall or both genes).

(Ren et al., 2005)

P{lacW}Jarid2^j2B8 homozygotes have eye defects. (FlyBase curator comment: it is not known whether this phenotype is caused by an effect on Jarid2 or pall or both genes).

(Berkeley Drosophila Genome Project, 1993.6.1)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

12078

y¹ w^*; P{lacW}Jarid2^j2B8/TM3, Sb¹

Notes on Origin

Discoverer

FlyBase curator comment: P{lacW}Jarid2^j2B8 results in loss of function of both the Jarid2 and pall genes (represented in FlyBase by the Jarid2^j2B8 and pall^j2B8 alleles respectively), and it has been shown that the phagocytosis-defective phenotype seen in P{lacW}Jarid2^j2B8 homozygotes is due to the loss of function of pall (FBrf0202092). The P{lacW}Jarid2^j2B8 chromosome also shows homozygous lethality, and it has been shown that the lethality maps to the insertion (FBrf0126832). A knock-out of the Jarid2 open reading frame is homozygous lethal (FBrf0236101), while a knock-out of the pall open reading frame is homozygous viable (FBrf0227703). This suggests that the lethality caused by the P{lacW}Jarid2^j2B8 insertion is due to an effect on Jarid2; information relating to the lethal phenotype (and the l(3)j2B8 symbol) has thus been assigned to the Jarid2^j2B8 allele in FlyBase.

(FlyBase, 1992-)

Genetic analysis indicates that the phagocytosis-defective phenotype seen in P{lacW}Jarid2^j2B8 homozygotes is caused by the insertion (precise excision of the insertion reverts this phenotype) and is due to the loss of function of pall (the phenotype can be fully rescued by expression of a UAS transgene encoding a full-length pall cDNA).

(Silva et al., 2007)

Comments

Reversion analysis shows that the P{lacW} insertion is responsible for the lethal phenotype.

(Beaton, 2000.1.31)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Jarid2^j2B8

l(3)j2B8

(Spradling et al., 1999)

l(3)j2B8^j2B8

Name Synonyms

Secondary FlyBase IDs

FBal0010916

References (6)

Report Sections

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