Insertion of TI{CRISPaint.T2A-GAL4} sequence into the 5' part of the wg coding sequence. The insertion is is in antisense orientation (T2A-GAL4 sequence within the inserted sequence cassette is in the opposite orientation to transcription of wg); as expected, no GAL4 expression is seen. An indel (33bp deletion, 11bp insertion) is associated with the site of the TI{CRISPaint.T2A-GAL4} insertion.
GGTACCCCCGA
Insertion of TI{CRISPaint.T2A-GAL4} sequence into the 5' part of the wg coding sequence. The insertion is is in antisense orientation (T2A-Scer\GAL4 sequence within the inserted sequence cassette is in the opposite orientation to transcription of wg). An indel (33bp deletion, 11bp insertion) is associated with the site of the TI{CRISPaint.T2A-GAL4} insertion.