FlyBase Allele Report: Dmel\strat[CRISPR]

General Information

Symbol

Dmel\strat^CRISPR

Species

D. melanogaster

Name

FlyBase ID

FBal0358351

Feature type

allele

Associated gene

Dmel\strat

Associated Insertion(s)

TI{TI}strat^CRISPR

Carried in Construct

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

CRISPR/Cas9

(Bellec et al., 2018)

gene targeting by homologous recombination

(Bellec et al., 2018)

Progenitor genotype

Associated Insertion(s)

TI{TI}strat^CRISPR

Cytology

Description

The first exon of the endogenous strat locus has been deleted and replaced by a sequence cassette that comprises an attP site followed by a loxP cassette containing a Disc\RFP^{DsRed.3xP3.cUa} marker.

(Bellec et al., 2018)

Allele components

Component

Use(s)

Also carries

attP

integrase target site

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell number | P-stage | somatic clone

(Bellec et al., 2018)

abnormal mitotic cell cycle | P-stage | somatic clone

(Bellec et al., 2018)

lethal - all die before end of larval stage

(Bellec et al., 2018)

lethal - all die before end of larval stage (with Df(2L)BSC200)

(Bellec et al., 2018)

Phenotype Manifest In

sensory neuron | P-stage | somatic clone

(Bellec et al., 2018)

sensory organ cell | P-stage | somatic clone

(Bellec et al., 2018)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

strat^CRISPR has abnormal cell number | P-stage | somatic clone phenotype, enhanceable by spdo^1.GFP

(Bellec et al., 2018)

Suppressed by

Statement

Reference

strat^CRISPR has abnormal cell number | somatic clone | P-stage phenotype, suppressible by Rab8^{Q67L.UASp.YFP}/Scer\GAL4^pnr.PU

(Bellec et al., 2018)

strat^CRISPR has abnormal cell number | somatic clone | P-stage phenotype, suppressible by Rab8^UASp.YFP/Scer\GAL4^pnr.PU

(Bellec et al., 2018)

NOT suppressed by

Statement

Reference

strat^CRISPR has abnormal cell number | somatic clone | P-stage phenotype, non-suppressible by Rab10^{Q68L.UASp.YFP}/Scer\GAL4^pnr.PU

(Bellec et al., 2018)

strat^CRISPR has abnormal cell number | somatic clone | P-stage phenotype, non-suppressible by Rab35^{Q67L.UASp.YFP}/Scer\GAL4^pnr.PU

(Bellec et al., 2018)

Phenotype Manifest In

Enhanced by

Statement

Reference

strat^CRISPR has sensory neuron | P-stage | somatic clone phenotype, enhanceable by spdo^1.GFP

(Bellec et al., 2018)

Suppressed by

Statement

Reference

strat^CRISPR has sensory neuron | somatic clone | P-stage phenotype, suppressible by Rab8^{Q67L.UASp.YFP}/Scer\GAL4^pnr.PU

(Bellec et al., 2018)

strat^CRISPR has sensory neuron | somatic clone | P-stage phenotype, suppressible by Rab8^UASp.YFP/Scer\GAL4^pnr.PU

(Bellec et al., 2018)

NOT suppressed by

Statement

Reference

strat^CRISPR has sensory neuron | somatic clone | P-stage phenotype, non-suppressible by Rab35^{Q67L.UASp.YFP}/Scer\GAL4^pnr.PU

(Bellec et al., 2018)

strat^CRISPR has sensory neuron | somatic clone | P-stage phenotype, non-suppressible by Rab10^{Q68L.UASp.YFP}/Scer\GAL4^pnr.PU

(Bellec et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

strat^CRISPR is rescued by strat^UASp.Tag:HA/Scer\GAL4^pnr.PU

(Bellec et al., 2018)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

strat^CRISPR

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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