FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Hnf4R167W.S169A.UAS.Tag:HA
Open Close
General Information
Symbol
Dmel\Hnf4R167W.S169A.UAS.Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0358398
Feature type
allele
Associated gene
Dmel\Hnf4
Associated Insertion(s)
Carried in Construct
M{UAS-Hnf4.R167W.S169A}
Key Links
Transgenic product class
missense_variant
(Marchesin et al., 2019)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Marchesin et al., 2019)
Mutagen
in vitro construct
(Marchesin et al., 2019)
Progenitor genotype
Carried in construct
M{UAS-Hnf4.R167W.S169A}
Cytology
Description

UAS regulates expression of the Tag:HA-tagged Hnf4 ORF containing the R167W mutation, which corresponds to the R85W mutation in human 'HNF4A' (aka'MODY1') associated with Fanconi renotubular syndrome (FRTS). This construct also contains a S169A mutation, which is equivalent to S87A in human 'HNF4A' (aka'MODY1'), thus suppressing PKC-dependent phosphorylation at that position and thereby also suppressing Hnf4 nuclear export and consequent cytotoxic effects.

(Marchesin et al., 2019)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Hnf4
Tagged with
Tag:HA
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      This allele represents a human variant implicated in disease.
      (Marchesin et al., 2019)
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Third instar larval garland nephrocytes expressing Hnf4R167W.S169A.UAS.Tag:HA under the control of Scer\GAL4Ugt36A1.PK exhibit a severe increase in lipid droplet content (BODIPY staining), but normal ER distribution, as compared to controls; upon Scer\GAL4sns.GCN-driven expression, there are no changes in pericardial nephrocyte viability, as compared to controls.

      (Marchesin et al., 2019)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Hnf4R167W.S169A.UAS.Tag:HA
      Name Synonyms
      Secondary FlyBase IDs
        References (2)