Orc4^Y162C homozygotes show several bristle defects compared to controls: wing-margin bristles are disorganized and vary in length, and several thorax bristles are missing, namely scutellar bristles.

While Orc4^Y162C homozygotes ovarioles appear largely normal and egg chambers are identifiable through stage 14, stage 10 egg chambers show follicle-cells with disrupted patterning and significantly fewer nurse cells (along with a wider distribution in the number of nurse cell per egg chamber); the amplification of chorion genes is absent (i.e. lower Hoechst signal). Eggs laid by Orc4^Y162C homozygous females don't have dorsal appendages and appear watery and malformed when compared to controls.

Orc4^Y162C heterozygous females bearing germline clones lay much fewer eggs that still exhibit normal dorsal appendages; the resulting embryos show general morphological defects.